ARG81812
Mouse Thrombomodulin ELISA Kit
Mouse Thrombomodulin ELISA Kit for ELISA and Mouse
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81812-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81812-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81812-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81812-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81812-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81812-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81812-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81812-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81812-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81812-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81812-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81812 Mouse Thrombomodulin ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse Thrombomodulin in serum, plasma (heparin, EDTA, citrate), urine and cell culture supernatants. |
|---|---|
| Tested Reactivity | Ms |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | Thrombomodulin |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 31.25 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA, citrate), urine and cell culture supernatants. |
| Standard Range | 62.5 - 4000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 5.2%; Inter-Assay CV: 6.3% |
| Alternate Names | THRM; CD antigen CD141; THPH12; TM; Thrombomodulin; CD141; BDCA3; Fetomodulin; AHUS6 |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | THBD |
| Gene Full Name | thrombomodulin |
| Background | The protein encoded by this intronless gene is an endothelial-specific type I membrane receptor that binds thrombin. This binding results in the activation of protein C, which degrades clotting factors Va and VIIIa and reduces the amount of thrombin generated. Mutations in this gene are a cause of thromboembolic disease, also known as inherited thrombophilia. [provided by RefSeq, Jul 2008] |
| Function | Thrombomodulin is a specific endothelial cell receptor that forms a 1:1 stoichiometric complex with thrombin. This complex is responsible for the conversion of protein C to the activated protein C (protein Ca). Once evolved, protein Ca scissions the activated cofactors of the coagulation mechanism, factor Va and factor VIIIa, and thereby reduces the amount of thrombin generated. [UniProt] |
| Highlight | Related products: Thrombomodulin antibodies; Thrombomodulin ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | N-glycosylated. The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG81812 Mouse Thrombomodulin ELISA Kit standard curve image
ARG81812 Mouse Thrombomodulin ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81812 Mouse Thrombomodulin ELISA Kit User's manual |
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