ARG82347

Mouse TECK ELISA Kit

Mouse TECK ELISA Kit for ELISA and Mouse

Component

Cat No Component Name Package Temp
ARG82347-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG82347-002 Standard 2 X 10 ng/vial 4°C
ARG82347-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG82347-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG82347-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG82347-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG82347-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG82347-008 25X Wash buffer 20 ml 4°C
ARG82347-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG82347-010 STOP solution 10 ml (Ready to use) 4°C
ARG82347-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG82347 Mouse TECK ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse TECK in serum, plasma (EDTA, heparin) and cell culture supernatants.
Tested Reactivity Ms
Tested Application ELISA
Target Name TECK
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 15.6 pg/ml
Sample Type Serum, plasma (EDTA, heparin) and cell culture supernatants.
Standard Range 31.2 - 2000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 6.8%
Inter-Assay CV: 7.2%
Alternate Names Chemokine TECK; Ckb15; SCYA25; Thymus-expressed chemokine; Small-inducible cytokine A25; TECK; C-C motif chemokine 25

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 20300 Mouse CCL25

Swiss-port # O35903 Mouse C-C motif chemokine 25

Gene Symbol CCL25
Gene Full Name chemokine (C-C motif) ligand 25
Background This antimicrobial gene belongs to the subfamily of small cytokine CC genes. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The cytokine encoded by this gene displays chemotactic activity for dendritic cells, thymocytes, and activated macrophages but is inactive on peripheral blood lymphocytes and neutrophils. The product of this gene binds to chemokine receptor CCR9. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2014]
Function Potentially involved in T-cell development. Recombinant protein shows chemotactic activity on thymocytes, macrophages, THP-1 cells, and dendritics cells but is inactive on peripheral blood lymphocytes and neutrophils. Binds to CCR9. Isoform 2 is an antagonist of isoform 1. Binds to atypical chemokine receptor ACKR4 and mediates the recruitment of beta-arrestin (ARRB1/2) to ACKR4. [UniProt]
Cellular Localization Secreted. [UniProt]
Highlight Related products:
TECK antibodies; TECK ELISA Kits;
New ELISA data calculation tool:
Simplify the ELISA analysis by GainData

Images (1) Click the Picture to Zoom In

  • ARG82347 Mouse TECK ELISA Kit standard curve image

    ARG82347 Mouse TECK ELISA Kit results of a typical standard run with optical density reading at 450 nm.

Title Download Link
ARG82347 Mouse TECK ELISA kit User manual download icon Download