ARG82275
Mouse TDGF1 / Cripto 1 ELISA Kit
Mouse TDGF1 / Cripto 1 ELISA Kit for ELISA and Mouse
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG82275-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82275-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82275-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82275-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82275-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82275-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82275-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82275-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82275-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82275-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82275-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG82275 Mouse TDGF1 / Cripto 1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse TDGF1 / Cripto 1 in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Ms |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | TDGF1 / Cripto 1 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 31.25 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| Standard Range | 62.5 - 4000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 5.0% Inter-Assay CV: 5.9% |
| Alternate Names | CRGF; Teratocarcinoma-derived growth factor 1; Cripto-1 growth factor; CR; CRIPTO; Epidermal growth factor-like cripto protein CR1 |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | TDGF1 |
|---|---|
| Gene Full Name | teratocarcinoma-derived growth factor 1 |
| Background | This gene encodes an epidermal growth factor-related protein that contains a cripto, FRL-1, and cryptic domain. The encoded protein is an extracellular, membrane-bound signaling protein that plays an essential role in embryonic development and tumor growth. Mutations in this gene are associated with forebrain defects. Pseudogenes of this gene are found on chromosomes 2, 3, 6, 8, 19 and X. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Mar 2010] |
| Function | Could play a role in the determination of the epiblastic cells that subsequently give rise to the mesoderm. [UniProt] |
| Cellular Localization | Cell membrane; Lipid-anchor, GPI-anchor. Secreted. Note=Released from the cell membrane by GPI cleavage. [UniProt] |
| Highlight | Related products: TDGF1 antibodies; TDGF1 ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
Images (1) Click the Picture to Zoom In
-
ARG82275 Mouse TDGF1 / Cripto 1 ELISA Kit standard curve image
ARG82275 Mouse TDGF1 / Cripto 1 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG82275 Mouse TDGF1 / Cripto 1 ELISA Kit User manual |
 Download
|
