ARG81837
Mouse Surfactant protein D ELISA Kit
Mouse Surfactant protein D ELISA Kit for ELISA and Mouse
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81837-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81837-002 | Standard | 2 X 20 ng/vial | 4°C |
| ARG81837-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81837-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81837-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81837-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81837-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81837-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81837-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81837-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81837-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81837 Mouse Surfactant protein D ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse Surfactant protein D in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Ms |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | Surfactant protein D |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 156 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| Standard Range | 312.5 - 20000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 6.0%; Inter-Assay CV: 7.2% |
| Alternate Names | SP-D; COLEC7; Lung surfactant protein D; SFTP4; Pulmonary surfactant-associated protein D; PSP-D; Collectin-7 |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P50404 Mouse Pulmonary surfactant-associated protein D |
|---|---|
| Gene Symbol | SFTPD |
| Gene Full Name | surfactant protein D |
| Background | The protein encoded by this gene is part of the innate immune response, protecting the lungs against inhaled microorganisms and chemicals. The encoded protein may also be involved in surfactant metabolism. [provided by RefSeq, Jul 2015] |
| Function | Contributes to the lung's defense against inhaled microorganisms, organic antigens and toxins. Interacts with compounds such as bacterial lipopolysaccharides, oligosaccharides and fatty acids and modulates leukocyte action in immune response. May participate in the extracellular reorganization or turnover of pulmonary surfactant. Binds strongly maltose residues and to a lesser extent other alpha-glucosyl moieties. [UniProt] |
| Highlight | Related products: Surfactant protein D ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | The N-terminus is blocked. Hydroxylation on proline residues within the sequence motif, GXPG, is most likely to be 4-hydroxy as this fits the requirement for 4-hydroxylation in vertebrates. S-nitrosylation at Cys-35 and Cys-40 alters the quaternary structure which results in a pro-inflammatory chemoattractive signaling activity with macrophages. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG81837 Mouse Surfactant protein D ELISA Kit standard curve image
ARG81837 Mouse Surfactant protein D ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81837 Mouse Surfactant protein D ELISA Kit User's manual |
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