ARG82541
Mouse Semaphorin 6A ELISA Kit
Mouse Semaphorin 6A ELISA Kit for ELISA and Mouse
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG82541-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82541-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82541-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82541-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82541-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82541-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82541-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82541-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82541-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82541-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82541-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG82541 Mouse Semaphorin 6A ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse Semaphorin 6A in serum, plasma (EDTA, heparin, citrate) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Ms |
| Tested Application | ELISA |
| Target Name | Semaphorin 6A |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 50 pg/ml |
| Sample Type | Serum, plasma (EDTA, heparin, citrate) and cell culture supernatants. |
| Standard Range | 62.5 - 4000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 4.8% Inter-Assay CV: 6.7% |
| Alternate Names | VIA; Semaphorin VIA; Semaphorin-6A; SEMAQ; Semaphorin-6A-1; SEMA; Sema VIA; SEMA6A-1; HT018; SEMA6A1 |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | SEMA6A |
| Gene Full Name | sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6A |
| Background | The transmembrane semaphorin SEMA6A is expressed in developing neural tissue and is required for proper development of the thalamocortical projection (Leighton et al., 2001 [PubMed 11242070]).[supplied by OMIM, Feb 2011] |
| Function | Cell surface receptor for PLXNA2 that plays an important role in cell-cell signaling. Required for normal granule cell migration in the developing cerebellum. Promotes reorganization of the actin cytoskeleton and plays an important role in axon guidance in the developing central nervous system. Can act as repulsive axon guidance cue. Has repulsive action towards migrating granular neurons. May play a role in channeling sympathetic axons into the sympathetic chains and controlling the temporal sequence of sympathetic target innervation (By similarity). [UniProt] |
| Cellular Localization | Cell membrane; Single-pass type I membrane protein. [UniProt] |
| Highlight | Related products: Semaphorin antibodies; Semaphorin ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
Images (1) Click the Picture to Zoom In
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ARG82541 Mouse Semaphorin 6A ELISA Kit standard curve image
ARG82541 Mouse Semaphorin 6A ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG82541 Mouse Semaphorin 6A ELISA Kit User manual |
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