ARG81963

Mouse S100A13 ELISA Kit

Mouse S100A13 ELISA Kit for ELISA and Mouse

Component

Cat No Component Name Package Temp
ARG81963-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG81963-002 Standard 2 X 10 ng/vial 4°C
ARG81963-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG81963-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG81963-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG81963-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG81963-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG81963-008 25X Wash buffer 20 ml 4°C
ARG81963-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG81963-010 STOP solution 10 ml (Ready to use) 4°C
ARG81963-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG81963 Mouse S100A13 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse S100A13 in serum, plasma (heparin, EDTA) and cell culture supernatants.
Tested Reactivity Ms
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name S100A13
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 7.8 pg/ml
Sample Type Serum, plasma (heparin, EDTA) and cell culture supernatants.
Standard Range 15.6 - 1000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 5.6%; Inter-Assay CV: 6.5%
Alternate Names Protein S100-A13; S100 calcium-binding protein A13

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Gene Symbol S100A13
Gene Full Name S100 calcium binding protein A13
Background The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein is widely expressed in various types of tissues with a high expression level in thyroid gland. In smooth muscle cells, this protein co-expresses with other family members in the nucleus and in stress fibers, suggesting diverse functions in signal transduction. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
Function Plays a role in the export of proteins that lack a signal peptide and are secreted by an alternative pathway. Binds two calcium ions per subunit. Binds one copper ion. Binding of one copper ion does not interfere with calcium binding. Required for the copper-dependent stress-induced export of IL1A and FGF1. The calcium-free protein binds to lipid vesicles containing phosphatidylserine, but not to vesicles containing phosphatidylcholine (By similarity). [UniProt]
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S100A antibodies; S100A ELISA Kits;
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Images (1) Click the Picture to Zoom In

  • ARG81963 Mouse S100A13 ELISA Kit standard curve image

    ARG81963 Mouse S100A13 ELISA Kit results of a typical standard run with optical density reading at 450 nm.

Title Download Link
ARG81963 Mouse S100A13 ELISA Kit User's manual download icon Download