ARG81987
Mouse PON1 / paraoxonase 1 ELISA Kit
Mouse PON1 / paraoxonase 1 ELISA Kit for ELISA and Mouse
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81987-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81987-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81987-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81987-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81987-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81987-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81987-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81987-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81987-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81987-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81987-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81987 Mouse PON1 / paraoxonase 1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse PON1 / paraoxonase 1 in serum, plasma (heparin) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Ms |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | PON1 / paraoxonase 1 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 78 pg/ml |
| Sample Type | Serum, plasma (heparin) and cell culture supernatants. |
| Standard Range | 156 - 10000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 6.0%; Inter-Assay CV: 7.4% |
| Alternate Names | K-45; Aromatic esterase 1; MVCD5; EC 3.1.1.81; A-esterase 1; PON; Serum paraoxonase/arylesterase 1; ESA; Serum aryldialkylphosphatase 1; EC 3.1.8.1; EC 3.1.1.2; PON 1 |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | PON1 |
| Gene Full Name | paraoxonase 1 |
| Background | The enzyme encoded by this gene is an arylesterase that mainly hydrolyzes paroxon to produce p-nitrophenol. Paroxon is an organophosphorus anticholinesterase compound that is produced in vivo by oxidation of the insecticide parathion. Polymorphisms in this gene are a risk factor in coronary artery disease. The gene is found in a cluster of three related paraoxonase genes at 7q21.3. [provided by RefSeq, Oct 2008] |
| Function | Hydrolyzes the toxic metabolites of a variety of organophosphorus insecticides. Capable of hydrolyzing a broad spectrum of organophosphate substrates and lactones, and a number of aromatic carboxylic acid esters. Mediates an enzymatic protection of low density lipoproteins against oxidative modification and the consequent series of events leading to atheroma formation. [UniProt] |
| Highlight | Related products: PON1 antibodies; PON1 ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | Glycosylated. The signal sequence is not cleaved. Present in two forms, form B contains a disulfide bond, form A does not. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG81987 Mouse PON1 / paraoxonase 1 ELISA Kit standard curve image
ARG81987 Mouse PON1 / paraoxonase 1 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81987 Mouse PON1 / paraoxonase 1 ELISA Kit User manual |
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