ARG82344

Mouse PLTP ELISA Kit

Mouse PLTP ELISA Kit for ELISA and Mouse

Component

Cat No Component Name Package Temp
ARG82344-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG82344-002 Standard 2 X 50 ng/vial 4°C
ARG82344-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG82344-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG82344-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG82344-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG82344-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG82344-008 25X Wash buffer 20 ml 4°C
ARG82344-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG82344-010 STOP solution 10 ml (Ready to use) 4°C
ARG82344-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG82344 Mouse PLTP ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse PLTP in serum, plasma (EDTA, heparin) and cell culture supernatants.
Tested Reactivity Ms
Tested Application ELISA
Target Name PLTP
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 0.39 ng/ml
Sample Type Serum, plasma (EDTA, heparin) and cell culture supernatants.
Standard Range 0.78 - 50 ng/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 5.8%
Inter-Assay CV: 7.0%
Alternate Names Lipid transfer protein II; Phospholipid transfer protein; BPIFE; HDLCQ9

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 18830 Mouse PLTP

Swiss-port # P55065 Mouse Phospholipid transfer protein

Gene Symbol PLTP
Gene Full Name phospholipid transfer protein
Background The protein encoded by this gene is one of at least two lipid transfer proteins found in human plasma. The encoded protein transfers phospholipids from triglyceride-rich lipoproteins to high density lipoprotein (HDL). In addition to regulating the size of HDL particles, this protein may be involved in cholesterol metabolism. At least two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Function Facilitates the transfer of a spectrum of different lipid molecules, including diacylglycerol, phosphatidic acid, sphingomyelin, phosphatidylcholine, phosphatidylglycerol, cerebroside and phosphatidyl ethanolamine. Essential for the transfer of excess surface lipids from triglyceride-rich lipoproteins to HDL, thereby facilitating the formation of smaller lipoprotein remnants, contributing to the formation of LDL, and assisting in the maturation of HDL particles. PLTP also plays a key role in the uptake of cholesterol from peripheral cells and tissues that is subsequently transported to the liver for degradation and excretion. Two distinct forms of PLTP exist in plasma: an active form that can transfer PC from phospholipid vesicles to high-density lipoproteins (HDL), and an inactive form that lacks this capability. [UniProt]
Cellular Localization Secreted. [UniProt]
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  • ARG82344 Mouse PLTP ELISA Kit standard curve image

    ARG82344 Mouse PLTP ELISA Kit results of a typical standard run with optical density reading at 450 nm.

Title Download Link
ARG82344 Mouse PLTP ELISA Kit User manual download icon Download