ARG81927
Mouse Neutrophil Elastase ELISA Kit
Mouse Neutrophil Elastase ELISA Kit for ELISA and Mouse
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81927-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81927-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81927-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81927-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81927-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81927-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81927-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81927-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81927-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81927-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81927-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81927 Mouse Neutrophil Elastase ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse Neutrophil Elastase in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Ms |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | Neutrophil Elastase |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 15.6 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| Standard Range | 31.2 - 2000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 7.0%; Inter-Assay CV: 7.3% |
| Alternate Names | Neutrophil elastase; HNE; EC 3.4.21.37; Medullasin; NE; ELA2; GE; Bone marrow serine protease; SCN1; PMN-E; Human leukocyte elastase; HLE; Elastase-2; PMN elastase |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | ELANE |
| Gene Full Name | elastase, neutrophil expressed |
| Background | Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode the structurally similar proteins. The product of this gene hydrolyzes proteins within specialized neutrophil lysosomes, called azurophil granules, as well as proteins of the extracellular matrix following the protein's release from activated neutrophils. The enzyme may play a role in degenerative and inflammatory diseases by its proteolysis of collagen-IV and elastin of the extracellular matrix. This protein degrades the outer membrane protein A (OmpA) of E. coli as well as the virulence factors of such bacteria as Shigella, Salmonella and Yersinia. Mutations in this gene are associated with cyclic neutropenia and severe congenital neutropenia (SCN). This gene is clustered with other serine protease gene family members, azurocidin 1 and proteinase 3 genes, at chromosome 19pter. All 3 genes are expressed coordinately and their protein products are packaged together into azurophil granules during neutrophil differentiation. [provided by RefSeq, May 2009] |
| Function | Modifies the functions of natural killer cells, monocytes and granulocytes. Inhibits C5a-dependent neutrophil enzyme release and chemotaxis. [UniProt] |
| Highlight | Related products: Neutrophil Elastase antibodies; Neutrophil Elastase ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
Images (1) Click the Picture to Zoom In
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ARG81927 Mouse Neutrophil Elastase ELISA Kit standard curve image
ARG81927 Mouse Neutrophil Elastase ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81927 Mouse Neutrophil Elastase ELISA Kit User manual |
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