ARG82355

Mouse METRNL ELISA Kit

Mouse METRNL ELISA Kit for ELISA and Mouse

Component

Cat No Component Name Package Temp
ARG82355-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG82355-002 Standard 2 X 10 ng/vial 4°C
ARG82355-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG82355-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG82355-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG82355-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG82355-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG82355-008 25X Wash buffer 20 ml 4°C
ARG82355-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG82355-010 STOP solution 10 ml (Ready to use) 4°C
ARG82355-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG82355 Mouse METRNL ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse METRNL in serum, plasma (EDTA, heparin) and cell culture supernatants.
Tested Reactivity Ms
Tested Application ELISA
Target Name METRNL
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 31.25 pg/ml
Sample Type Serum, plasma (EDTA, heparin) and cell culture supernatants.
Standard Range 62.5 - 4000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 5.9%
Inter-Assay CV: 6.4%
Alternate Names Meteorin-like protein; Subfatin

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 210029 Mouse METRNL

Swiss-port # Q8VE43 Mouse Meteorin-like protein

Gene Symbol METRNL
Gene Full Name meteorin, glial cell differentiation regulator-like
Function Hormone induced following exercise or cold exposure that promotes energy expenditure. Induced either in the skeletal muscle after exercise or in adipose tissue following cold exposure and is present in the circulation. Able to stimulate energy expenditure associated with the browning of the white fat depots and improves glucose tolerance. Does not promote an increase in a thermogenic gene program via direct action on adipocytes, but acts by stimulating several immune cell subtypes to enter the adipose tissue and activate their prothermogenic actions. Stimulates an eosinophil-dependent increase in IL4 expression and promotes alternative activation of adipose tissue macrophages, which are required for the increased expression of the thermogenic and anti-inflammatory gene programs in fat. Required for some cold-induced thermogenic responses, suggesting a role in metabolic adaptations to cold temperatures (By similarity). [UniProt]
Cellular Localization Secreted. [UniProt]
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  • ARG82355 Mouse METRNL ELISA Kit standard curve image

    ARG82355 Mouse METRNL ELISA Kit results of a typical standard run with optical density reading at 450 nm.

Title Download Link
ARG82355 Mouse METRNL ELISA kit User manual download icon Download