ARG81864
Mouse Lumican ELISA Kit
Mouse Lumican ELISA Kit for ELISA and Mouse
Publication1
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81864-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81864-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81864-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81864-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81864-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81864-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81864-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81864-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81864-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81864-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81864-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81864 Mouse Lumican ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse Lumican in serum, plasma (heparin) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Ms |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | Lumican |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 78 pg/ml |
| Sample Type | Serum, plasma (heparin) and cell culture supernatants. |
| Standard Range | 156 - 10000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 6.5%; Inter-Assay CV: 7.9% |
| Alternate Names | LDC; Keratan sulfate proteoglycan lumican; Lumican; SLRR2D; KSPG lumican |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | LUM |
| Gene Full Name | lumican |
| Background | This gene encodes a member of the small leucine-rich proteoglycan (SLRP) family that includes decorin, biglycan, fibromodulin, keratocan, epiphycan, and osteoglycin. In these bifunctional molecules, the protein moiety binds collagen fibrils and the highly charged hydrophilic glycosaminoglycans regulate interfibrillar spacings. Lumican is the major keratan sulfate proteoglycan of the cornea but is also distributed in interstitial collagenous matrices throughout the body. Lumican may regulate collagen fibril organization and circumferential growth, corneal transparency, and epithelial cell migration and tissue repair. [provided by RefSeq, Jul 2008] |
| Highlight | Related products: Lumican antibodies; Lumican ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | Sulfated on tyrosine residue(s). [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG81864 Mouse Lumican ELISA Kit standard curve image
ARG81864 Mouse Lumican ELISA Kit results of a typical standard run with optical density reading at 450 nm.
Specific References
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ELISA / Mouse / Cell culture supernatants
| Title | Download Link |
|---|---|
| ARG81864 Mouse Lumican ELISA Kit User manual |
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