ARG82354
Mouse KREMEN1 ELISA Kit
Mouse KREMEN1 ELISA Kit for ELISA and Mouse
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG82354-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82354-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82354-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82354-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82354-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82354-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82354-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82354-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82354-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82354-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82354-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG82354 Mouse KREMEN1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse KREMEN1 in serum, plasma (EDTA, heparin) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Ms |
| Tested Application | ELISA |
| Target Name | KREMEN1 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 62.5 pg/ml |
| Sample Type | Serum, plasma (EDTA, heparin) and cell culture supernatants. |
| Standard Range | 125 - 8000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 6.1% Inter-Assay CV: 6.7% |
| Alternate Names | KREMEN; KRM1; Dickkopf receptor; Kringle domain-containing transmembrane protein 1; Kremen protein 1; Kringle-containing protein marking the eye and the nose |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | KREMEN1 |
| Gene Full Name | kringle containing transmembrane protein 1 |
| Background | This gene encodes a high-affinity dickkopf homolog 1 (DKK1) transmembrane receptor that functionally cooperates with DKK1 to block wingless (WNT)/beta-catenin signaling. The encoded protein is a component of a membrane complex that modulates canonical WNT signaling through lipoprotein receptor-related protein 6 (LRP6). It contains extracellular kringle, WSC, and CUB domains. Alternatively spliced transcript variants encoding distinct isoforms have been observed for this gene. [provided by RefSeq, Jul 2008] |
| Function | Receptor for Dickkopf protein. Cooperates with Dickkopf to block Wnt/beta-catenin signaling (By similarity). [UniProt] |
| Cellular Localization | Cell membrane; Single-pass type I membrane protein. [UniProt] |
| Highlight | Related products: KREMEN1 antibodies; KREMEN1 ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
Images (1) Click the Picture to Zoom In
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ARG82354 Mouse KREMEN1 ELISA Kit standard curve image
ARG82354 Mouse KREMEN1 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG82354 Mouse KREMEN1 ELISA Kit User manual |
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