ARG81975
Mouse KLK1 ELISA Kit
Mouse KLK1 ELISA Kit for ELISA and Mouse
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81975-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81975-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81975-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81975-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81975-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81975-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81975-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81975-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81975-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81975-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81975-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81975 Mouse KLK1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse KLK1 in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Ms |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | Kallikrein 1 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 31.25 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| Standard Range | 62.5 - 4000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 7.1%; Inter-Assay CV: 8.2% |
| Alternate Names | EC 3.4.21.35; Tissue kallikrein; hK1; Kallikrein-1; KLKR; Kidney/pancreas/salivary gland kallikrein; Klk6 |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | KLK1 |
| Gene Full Name | kallikrein 1 |
| Background | Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. This protein is functionally conserved in its capacity to release the vasoactive peptide, Lys-bradykinin, from low molecular weight kininogen. [provided by RefSeq, Jul 2008] |
| Function | Glandular kallikreins cleave Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin. [UniProt] |
| Highlight | Related products: Kallikrein antibodies; Kallikrein ELISA Kits; Kallikrein Duos / Panels; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | The O-linked polysaccharides on Ser-93, Ser-104 and Ser-167 are probably the mucin type linked to GalNAc. In PubMed:3163150, GalNAc was detected with the corresponding peptides but not located. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG81975 Mouse KLK1 ELISA Kit standard curve image
ARG81975 Mouse KLK1 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81975 Mouse KLK1 ELISA Kit User's manual |
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