ARG65630

Mouse IgG2a Isotype Control antibody [MOPC-173] (APC)

Mouse IgG2a Isotype Control antibody [MOPC-173] (APC) for Flow cytometry

Controls and Markers antibody

Overview

Product Description APC-conjugated Mouse Monoclonal antibody [MOPC-173] as a negative control antibody for Mouse IgG2a
Species Does Not React With Hu, Ms, Rat
Tested Application FACS
Specificity This mouse IgG2a monoclonal antibody (clone MOPC-173) reacts with an unknown epitope. It does not react with a variety of resting, activated, live, and fixed mouse, rat and human tissues.
Host Mouse
Clonality Monoclonal
Clone MOPC-173
Isotype IgG2a
Target Name Mouse IgG2a
Immunogen The transplantable plasmacytoma MOPC-173 was induced by intraperitoneal injection of mineral oils into BALB/c mice.
Conjugation APC

Application Instructions

Application Suggestion
Tested Application Dilution
FACS8 - 10 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Buffer PBS, 15 mM Sodium azide and 0.2% (w/v) high-grade protease free BSA
Preservative 15 mM Sodium azide
Stabilizer 0.2% BSA
Concentration 0.1 mg/ml
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Background The specificity of staining by monoclonal antibodies to target antigens should be verified by establishing the amount of non-specific antibody binding. Especially at higher concentration (more than 15 μg/ml) the antibody staining usually has consignable background. To this end a non-reactive immunoglobulin of the same isotype is included as a negative control for each specific monoclonal antibody used in a particular immunoassay.
Research Area Controls and Markers antibody

Images (1) Click the Picture to Zoom In

  • ARG65630 Mouse IgG2a Isotype Control antibody [MOPC-173] (APC) FACS image

    Flow Cytometry: Human peripheral blood stained with ARG65630 Mouse IgG2a Isotype Control antibody [MOPC-173] (APC) at 9 µg/ml dilution.