ARG80216
Mouse IgE ELISA Kit
Mouse IgE ELISA Kit for ELISA and Mouse
Immune System kit
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG80216-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG80216-002 | Standard (Lyophilized) | 3 X 80 ng/vial | 4°C |
| ARG80216-003 | Standard diluent buffer | 40 ml | 4°C |
| ARG80216-004 | Antibody conjugate concentrate | 400 µl | 4°C |
| ARG80216-005 | Antibody diluent buffer | 16 ml | 4°C |
| ARG80216-006 | 20X Wash buffer | 50 ml | 4°C |
| ARG80216-007 | TMB substrate | 12ml | 4°C (Protect from light) |
| ARG80216-008 | STOP solution | 12ml | 4°C |
| ARG80216-009 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG80216 Mouse IgE ELISA Kit is a Enzyme Immunoassay kit for the quantification of Mouse Mouse IgE in Serum, Plasma, Cell culture supernatants |
|---|---|
| Tested Reactivity | Ms |
| Tested Application | ELISA |
| Specificity | No significant cross-reactivity or interference with Mouse IL-1,2,3,5,6,8,10,IFN,TNF；human IgE；rat IgE |
| Target Name | IgE |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm |
| Sensitivity | 0.8 ng/ml |
| Sample Type | Serum, Plasma, Cell culture supernatants |
| Standard Range | 1.56 - 100 ng/ml |
| Sample Volume | 100 µl |
| Precision | CV: <10% |
| Alternate Names | Immunoglobulin E |
Application Instructions
| Assay Time | 4 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Background | Immunoglobulin E (IgE) is a 180 kDa soluble protein serving as an antigen-specific unit of mast cell effector mechanisms. IgE has the lowest serum concentration of all immunoglobulins (approximately 0.5 mg/l) in healthy individuals, but upon allergen challenge its concentration in blood increases dramatically. Although biological survival of free IgE is very short (T1/2 = 2 days), it is stabilized after binding to its high affinity receptor. Unlike IgM- IgG- and IgA-committed B cells, IgE-switched B cells do not undergo clonal expansion. |
|---|---|
| Highlight | Related products: IgE ELISA Kits; Related news: A designer cell-based histamine-specific human allergy profiler New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| Research Area | Immune System kit |
Images (1) Click the Picture to Zoom In
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ARG80216 Mouse IgE ELISA Kit ELISA Kit standard curve image
ARG80216 Mouse IgE ELISA Kit ELISA Kit results of a typical standard run with optical density reading at 450nm.
| Title | Download Link |
|---|---|
| ARG80216 Mouse IgE ELISA Kit User's manual |
 Download
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