ARG81805
Mouse IL31 ELISA Kit
Mouse IL31 ELISA Kit for ELISA and Mouse
Publication1
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81805-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81805-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81805-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81805-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81805-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81805-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81805-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81805-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81805-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81805-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81805-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81805 Mouse IL31 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse IL31 in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Ms |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | IL31 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 31.25 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| Standard Range | 62.5 - 4000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 5.6%; Inter-Assay CV: 6.3% |
| Alternate Names | IL-31; Interleukin-31 |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | IL31 |
| Gene Full Name | interleukin 31 |
| Background | IL31, which is made principally by activated Th2-type T cells, interacts with a heterodimeric receptor consisting of IL31RA (MIM 609510) and OSMR (MIM 601743) that is constitutively expressed on epithelial cells and keratinocytes. IL31 may be involved in the promotion of allergic skin disorders and in regulating other allergic diseases, such as asthma (Dillon et al., 2004 [PubMed 15184896]).[supplied by OMIM, Mar 2008] |
| Function | Activates STAT3 and possibly STAT1 and STAT5 through the IL31 heterodimeric receptor composed of IL31RA and OSMR. IL31 may function in skin immunity. [UniProt] |
| Highlight | Related products: IL31 antibodies; IL31 ELISA Kits; IL31 recombinant proteins; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
Images (1) Click the Picture to Zoom In
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ARG81805 Mouse IL31 ELISA Kit standard curve image
ARG81805 Mouse IL31 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
Specific References
Calcium-Based Antimicrobial Peptide Compounds Attenuate DNFB-Induced Atopic Dermatitis-Like Skin Lesions via Th-Cells in BALB/c Mice
ELISA / Mouse / Serum
| Title | Download Link |
|---|---|
| ARG81805 Mouse IL31 ELISA Kit User's manual |
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