ARG70155
Mouse IL3 recombinant protein (Active) (His-tagged, C-ter)
Mouse IL3 recombinant protein (Active) (His-tagged, C-ter) for SDS-PAGE
Overview
| Product Description | E. coli expressed, His-tagged (C-ter) Active Mouse IL3 recombinant protein |
|---|---|
| Tested Application | SDS-PAGE |
| Target Name | IL3 |
| Species | Mouse |
| A.A. Sequence | Asp33 - Cys166 |
| Expression System | E. coli |
| Activity | Active |
| Activity Note | Determined by its ability to induce NFS-60 cells proliferation. The ED50 for this effect is <85 pg/mL. The specific activity of recombinant mouse IL-3 is approximately >1x 10^7 IU/mg. |
| Alternate Names | MCGF; Mast cell growth factor; P-cell-stimulating factor; Hematopoietic growth factor; IL-3; Interleukin-3; Multipotential colony-stimulating factor; MULTI-CSF |
Properties
| Form | Powder |
|---|---|
| Purification Note | Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test. |
| Purity | >95% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) |
| Reconstitution | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely. |
| Storage Instruction | For long term, lyophilized protein should be stored at -20°C or -80°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | IL3 |
|---|---|
| Gene Full Name | interleukin 3 |
| Background | The protein encoded by this gene is a potent growth promoting cytokine. This cytokine is capable of supporting the proliferation of a broad range of hematopoietic cell types. It is involved in a variety of cell activities such as cell growth, differentiation and apoptosis. This cytokine has been shown to also possess neurotrophic activity, and it may be associated with neurologic disorders. [provided by RefSeq, Jul 2008] |
| Function | Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes, macrophages, mast cells, stem cells, erythroid cells, eosinophils and megakaryocytes. [UniProt] |
| Cellular Localization | Secreted. [UniProt] |
Images (1) Click the Picture to Zoom In
-
ARG70155 Mouse IL3 recombinant protein (Active) (His-tagged, C-ter) SDS-PAGE image
SDS-PAGE analysis of ARG70155 Mouse IL3 recombinant protein (Active) (His-tagged, C-ter).
