ARG70175
Mouse IL25 / IL17E recombinant protein (Active) (His-tagged, C-ter)
Mouse IL25 / IL17E recombinant protein (Active) (His-tagged, C-ter) for SDS-PAGE
Overview
| Product Description | E. coli expressed, His-tagged (C-ter) Active Mouse IL25 / IL17E recombinant protein |
|---|---|
| Tested Application | SDS-PAGE |
| Target Name | IL25 / IL17E |
| Species | Mouse |
| A.A. Sequence | Val17 - Ala169 |
| Expression System | E. coli |
| Activity | Active |
| Activity Note | Determinedd by its ability to induce CXCL1 secretion in HT‑29 cells. The ED50 for this effect is <1 ng/mL |
| Alternate Names | IL17E; Interleukin-17E; IL-25; Interleukin-25; IL-17E |
Properties
| Form | Powder |
|---|---|
| Purification Note | Endotoxin level is <0.1 EU/µg of the protein, as determined by the LAL test. |
| Purity | >98% (by SDS-PAGE) |
| Buffer | 20 mM sodium citrate and 0.2 M NaCl (pH 4.5) |
| Reconstitution | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not <200 μg/mL and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely. |
| Storage Instruction | For long term, lyophilized protein should be stored at -20°C or -80°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | IL25 |
|---|---|
| Gene Full Name | interleukin 25 |
| Background | The protein encoded by this gene is a cytokine that shares sequence similarity with interleukin 17. This cytokine can induce NF-kappaB activation, and stimulate the production of interleukin 8. Both this cytokine and interleukin 17B are ligands for the cytokine receptor IL17BR. Studies of a similar gene in mice suggest that this cytokine may be a pro-inflammatory cytokine favoring the Th2-type immune response. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2010] |
| Function | Induces activation of NF-kappa-B and stimulates production of the proinflammatory chemokine IL-8. Proinflammatory cytokine favoring Th2-type immune responses. [UniProt] |
| Cellular Localization | Secreted. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG70175 Mouse IL25 / IL17E recombinant protein (Active) (His-tagged, C-ter) SDS-PAGE image
SDS-PAGE analysis of ARG70175 Mouse IL25 / IL17E recombinant protein (Active) (His-tagged, C-ter).
