ARG81900
Mouse IL24 ELISA Kit
Mouse IL24 ELISA Kit for ELISA and Mouse
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81900-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81900-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81900-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81900-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81900-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81900-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81900-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81900-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81900-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81900-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81900-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81900 Mouse IL24 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse IL24 in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Ms |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | IL24 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 31.25 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| Standard Range | 62.5 - 4000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 5.2%; Inter-Assay CV: 5.8% |
| Alternate Names | IL10B; Interleukin-24; Melanoma differentiation-associated gene 7 protein; MDA7; MDA-7; C49A; ST16; FISP; Suppression of tumorigenicity 16 protein; IL-24; MOB5 |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | IL24 |
| Gene Full Name | interleukin 24 |
| Background | This gene encodes a member of the IL10 family of cytokines. It was identified as a gene induced during terminal differentiation in melanoma cells. The protein encoded by this gene can induce apoptosis selectively in various cancer cells. Overexpression of this gene leads to elevated expression of several GADD family genes, which correlates with the induction of apoptosis. The phosphorylation of mitogen-activated protein kinase 14 (MAPK7/P38), and heat shock 27kDa protein 1 (HSPB2/HSP27) are found to be induced by this gene in melanoma cells, but not in normal immortal melanocytes. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008] |
| Function | Has antiproliferative properties on melanoma cells and may contribute to terminal cell differentiation. [UniProt] |
| Highlight | Related products: IL24 antibodies; IL24 ELISA Kits; IL24 recombinant proteins; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | Ubiquitination at Lys-122 promotes proteasomal degradation. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG81900 Mouse IL24 ELISA Kit standard curve image
ARG81900 Mouse IL24 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81900 Mouse IL24 ELISA Kit User's manual |
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