ARG70169
Mouse IL18 recombinant protein (Active) (His-tagged, C-ter)
Mouse IL18 recombinant protein (Active) (His-tagged, C-ter) for SDS-PAGE
Overview
| Product Description | E. coli expressed, His-tagged (C-ter) Active Mouse IL18 recombinant protein |
|---|---|
| Tested Application | SDS-PAGE |
| Target Name | IL18 |
| Species | Mouse |
| A.A. Sequence | Asn36 - Ser192 |
| Expression System | E. coli |
| Activity | Active |
| Activity Note | Determined by its ability to induce IFN gamma secretion in KG-1 cells. The ED50 for this effect is <0.5 µg/mL. |
| Alternate Names | Iboctadekin; Interferon gamma-inducing factor; Interleukin-18; IL-18; IFN-gamma-inducing factor; IL-1 gamma; IGIF; IL-1g; Interleukin-1 gamma; IL1F4 |
Properties
| Form | Powder |
|---|---|
| Purification Note | Endotoxin level is <0.1 EU/µg of the protein, as determined by the LAL test. |
| Purity | >98% (by SDS-PAGE) |
| Buffer | PBS (pH 8.0) |
| Reconstitution | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not <200 μg/mL and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely. |
| Storage Instruction | For long term, lyophilized protein should be stored at -20°C or -80°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | IL18 |
|---|---|
| Gene Full Name | interleukin 18 |
| Background | The protein encoded by this gene is a proinflammatory cytokine that augments natural killer cell activity in spleen cells, and stimulates interferon gamma production in T-helper type I cells. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Aug 2011] |
| Function | Augments natural killer cell activity in spleen cells and stimulates interferon gamma production in T-helper type I cells. [UniProt] |
| Cellular Localization | Secreted. [UniProt] |
| PTM | The pro-IL-18 precursor is processed by CASP1 or CASP4 to yield the active form. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG70169 Mouse IL18 recombinant protein (Active) (His-tagged, C-ter) SDS-PAGE image
SDS-PAGE analysis of ARG70169 Mouse IL18 recombinant protein (Active) (His-tagged, C-ter).
