ARG81819

Mouse IL15RA ELISA Kit

Mouse IL15RA ELISA Kit for ELISA and Mouse

Component

Cat No Component Name Package Temp
ARG81819-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG81819-002 Standard 2 X 10 ng/vial 4°C
ARG81819-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG81819-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG81819-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG81819-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG81819-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG81819-008 25X Wash buffer 20 ml 4°C
ARG81819-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG81819-010 STOP solution 10 ml (Ready to use) 4°C
ARG81819-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG81819 Mouse IL15RA ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse IL15RA in serum, plasma (heparin, EDTA) and cell culture supernatants.
Tested Reactivity Ms
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name IL15RA
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 62.5 pg/ml
Sample Type Serum, plasma (heparin, EDTA) and cell culture supernatants.
Standard Range 125 - 8000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 6.3%; Inter-Assay CV: 6.8%
Alternate Names sIL-15 receptor subunit alpha; sIL-15RA; Interleukin-15 receptor subunit alpha; IL-15R-alpha; IL-15RA; IL-15 receptor subunit alpha; CD antigen CD215; CD215; sIL-15R-alpha

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 16169 Mouse IL15RA

Swiss-port # Q60819 Mouse Interleukin-15 receptor subunit alpha

Gene Symbol IL15RA
Gene Full Name interleukin 15 receptor, alpha
Background This gene encodes a cytokine receptor that specifically binds interleukin 15 (IL15) with high affinity. The receptors of IL15 and IL2 share two subunits, IL2R beta and IL2R gamma. This forms the basis of many overlapping biological activities of IL15 and IL2. The protein encoded by this gene is structurally related to IL2R alpha, an additional IL2-specific alpha subunit necessary for high affinity IL2 binding. Unlike IL2RA, IL15RA is capable of binding IL15 with high affinity independent of other subunits, which suggests distinct roles between IL15 and IL2. This receptor is reported to enhance cell proliferation and expression of apoptosis inhibitor BCL2L1/BCL2-XL and BCL2. Multiple alternatively spliced transcript variants of this gene have been reported.[provided by RefSeq, Apr 2010]
Function High-affinity receptor for interleukin-15. Can signal both in cis and trans where IL15R from one subset of cells presents IL15 to neighboring IL2RG-expressing cells. Expression of different isoforms may alter or interfere with signal transduction. Isoform 5, isoform 6, isoform 7 and isoform 8 do not bind IL15. Signal transduction involves SYK. [UniProt]
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PTM A soluble form (sIL-15RA) arises from proteolytic shedding of the membrane-anchored receptor. The cleavage involves ADAM17/TACE (By similarity). It also binds IL-15 and thus interferes with IL-15 binding to the membrane receptor.

Phosphorylated by activated SYK.

N-glycosylated and O-glycosylated. [UniProt]

Images (1) Click the Picture to Zoom In

  • ARG81819 Mouse IL15RA ELISA Kit standard curve image

    ARG81819 Mouse IL15RA ELISA Kit results of a typical standard run with optical density reading at 450 nm.

Title Download Link
ARG81819 Mouse IL15RA ELISA Kit User's manual download icon Download