ARG82459
Mouse IFNAR1 ELISA Kit
Mouse IFNAR1 ELISA Kit for ELISA and Mouse
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG82459-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82459-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82459-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82459-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82459-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82459-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82459-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82459-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82459-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82459-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82459-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG82459 Mouse IFNAR1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse IFNAR1 in serum, plasma (EDTA, heparin) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Ms |
| Tested Application | ELISA |
| Target Name | IFNAR1 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 31.25 pg/ml |
| Sample Type | Serum, plasma (EDTA, heparin) and cell culture supernatants. |
| Standard Range | 62.5 - 4000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 4.3% Inter-Assay CV: 5.8% |
| Alternate Names | Cytokine receptor class-II member 1; Cytokine receptor family 2 member 1; IFRC; IFNAR; AVP; IFN-alpha/beta receptor 1; CRF2-1; IFN-R-1; Type I interferon receptor 1; IFNBR; Interferon alpha/beta receptor 1; IFN-alpha-REC |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | IFNAR1 |
| Gene Full Name | interferon (alpha, beta and omega) receptor 1 |
| Background | The protein encoded by this gene is a type I membrane protein that forms one of the two chains of a receptor for interferons alpha and beta. Binding and activation of the receptor stimulates Janus protein kinases, which in turn phosphorylate several proteins, including STAT1 and STAT2. The encoded protein also functions as an antiviral factor. [provided by RefSeq, Jul 2008] |
| Function | Associates with IFNAR2 to form the type I interferon receptor. Receptor for interferons alpha and beta. Binding to type I IFNs triggers tyrosine phosphorylation of a number of proteins including JAKs, TYK2, STAT proteins and IFNR alpha- and beta-subunits themselves. Can also transduce IFNB signals without the help of IFNAR2, and not activating the Jak-STAT pathway. [UniProt] |
| Cellular Localization | Isoform 1: Cell membrane; Single-pass type I membrane protein. Late endosome. Lysosome. Note=Interferon binding triggers internalization of the receptor from the cell membrane into endosomes and then into lysosomes. [UniProt] |
| Highlight | Related products: IFNAR1 antibodies; IFNAR1 ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | Ubiquitinated, leading to its internalization and degradation (PubMed:14532120, PubMed:15337770). Polyubiquitinated via 'Lys-48'-linked and 'Lys-63'-linked ubiquitin chains, leading to receptor internalization and lysosomal degradation (PubMed:18056411). The 'Lys-63'-linked ubiquitin chains are cleaved off by the BRISC complex (PubMed:24075985). Phosphorylated on serine residues in response to interferon binding; this promotes interaction with FBXW11 and ubiquitination (PubMed:14532120, PubMed:15337770, PubMed:24075985). Phosphorylated on tyrosine residues by TYK2 tyrosine kinase (PubMed:7526154). Phosphorylated on tyrosine residues in response to interferon (PubMed:10049744). Palmitoylation at Cys-463 is required for the activation of STAT1 and STAT2. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG82459 Mouse IFNAR1 ELISA Kit standard curve image
ARG82459 Mouse IFNAR1 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG82459 Mouse IFNAR1 ELISA Kit User manual |
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