ARG82369
Mouse Haptoglobin ELISA Kit
Mouse Haptoglobin ELISA Kit for ELISA and Mouse
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG82369-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82369-002 | Standard | 2 X 100 ng/vial | 4°C |
| ARG82369-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82369-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82369-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82369-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82369-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82369-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82369-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82369-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82369-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG82369 Mouse Haptoglobin ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse Haptoglobin in serum, plasma (EDTA, heparin) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Ms |
| Tested Application | ELISA |
| Target Name | Haptoglobin |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 0.78 ng/ml |
| Sample Type | Serum, plasma (EDTA, heparin) and cell culture supernatants. |
| Standard Range | 1.56 - 100 ng/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 6.6% Inter-Assay CV: 6.9% |
| Alternate Names | HPA1S; Haptoglobin; BP; HP2ALPHA2; Zonulin |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | HP |
| Gene Full Name | haptoglobin |
| Background | This gene encodes a preproprotein, which is processed to yield both alpha and beta chains, which subsequently combine as a tetramer to produce haptoglobin. Haptoglobin functions to bind free plasma hemoglobin, which allows degradative enzymes to gain access to the hemoglobin, while at the same time preventing loss of iron through the kidneys and protecting the kidneys from damage by hemoglobin. Mutations in this gene and/or its regulatory regions cause ahaptoglobinemia or hypohaptoglobinemia. This gene has also been linked to diabetic nephropathy, the incidence of coronary artery disease in type 1 diabetes, Crohn's disease, inflammatory disease behavior, primary sclerosing cholangitis, susceptibility to idiopathic Parkinson's disease, and a reduced incidence of Plasmodium falciparum malaria. The protein encoded also exhibits antimicrobial activity against bacteria. A similar duplicated gene is located next to this gene on chromosome 16. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2014] |
| Function | As a result of hemolysis, hemoglobin is found to accumulate in the kidney and is secreted in the urine. Haptoglobin captures, and combines with free plasma hemoglobin to allow hepatic recycling of heme iron and to prevent kidney damage. Haptoglobin also acts as an Antimicrobial; Antioxidant, has antibacterial activity and plays a role in modulating many aspects of the acute phase response. Hemoglobin/haptoglobin complexes are rapidely cleared by the macrophage CD163 scavenger receptor expressed on the surface of liver Kupfer cells through an endocytic lysosomal degradation pathway. Uncleaved haptoglogin, also known as zonulin, plays a role in intestinal permeability, allowing intercellular tight junction disassembly, and controlling the equilibrium between tolerance and immunity to non-self antigens. [UniProt] |
| Cellular Localization | Secreted. [UniProt] |
| Highlight | Related products: Haptoglobin antibodies; Haptoglobin ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
Images (1) Click the Picture to Zoom In
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ARG82369 Mouse Haptoglobin ELISA Kit standard curve image
ARG82369 Mouse Haptoglobin ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG82369 Mouse Haptoglobin ELISA Kit User manual |
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