ARG70458
Mouse GDNF recombinant protein (His-tagged, C-ter)
Mouse GDNF recombinant protein (His-tagged, C-ter) for SDS-PAGE
Overview
| Product Description | E. coli expressed, His-tagged (C-ter) Mouse GDNF recombinant protein |
|---|---|
| Tested Application | SDS-PAGE |
| Target Name | GDNF |
| Species | Mouse |
| A.A. Sequence | Ser78 - Ile211 |
| Expression System | E. coli |
| Protein Full Name | Glial cell line-derived neurotrophic factor |
| Alternate Names | GDNF; Glial Cell Derived Neurotrophic Factor; Astrocyte-Derived Trophic Factor; HFB1-GDNF; ATF1; ATF2 |
Properties
| Form | Powder |
|---|---|
| Purification Note | Endotoxin level is <0.1 EU/µg of the protein, as determined by the LAL test. |
| Purity | >98% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) |
| Reconstitution | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not <200 μg/mL and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely. |
| Storage Instruction | For long term, lyophilized protein should be stored at -20°C or -80°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | GDNF |
|---|---|
| Gene Full Name | Glial Cell Derived Neurotrophic Factor |
| Background | This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. The recombinant form of this protein, a highly conserved neurotrophic factor, was shown to promote the survival and differentiation of dopaminergic neurons in culture, and was able to prevent apoptosis of motor neurons induced by axotomy. This protein is a ligand for the product of the RET (rearranged during transfection) protooncogene. Mutations in this gene may be associated with Hirschsprung disease and Tourette syndrome. This gene encodes multiple protein isoforms that may undergo similar proteolytic processing. |
| Function | Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake. |
| Cellular Localization | Secreted |
| PTM | Cleavage on pair of basic residues, Disulfide bond, Glycoprotein |
Images (1) Click the Picture to Zoom In
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ARG70458 Mouse GDNF recombinant protein (His-tagged, C-ter) SDS-PAGE image
SDS-PAGE analysis of ARG70458 Mouse GDNF recombinant protein (His-tagged, C-ter)
