ARG82731
Mouse ERAP1 ELISA Kit
Mouse ERAP1 ELISA Kit for ELISA and Mouse
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG82731-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82731-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82731-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82731-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82731-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82731-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82731-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82731-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82731-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82731-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82731-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG82731 Mouse ERAP1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse ERAP1 in serum, plasma (EDTA, heparin) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Ms |
| Tested Application | ELISA |
| Target Name | ERAP1 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 156 pg/ml |
| Sample Type | Serum, plasma (EDTA, heparin) and cell culture supernatants. |
| Standard Range | 312 - 20000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 5.5% Inter-Assay CV: 6.8% |
| Alternate Names | Puromycin-insensitive leucyl-specific aminopeptidase; PILS-AP; EC 3.4.11.-; ARTS1; Adipocyte-derived leucine aminopeptidase; ERAAP1; ARTS-1; A-LAP; PILSAP; ALAP; Endoplasmic reticulum aminopeptidase 1; Aminopeptidase PILS; Type 1 tumor necrosis factor receptor shedding aminopeptidase regulator; APPILS; ERAAP |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q9EQH2 Mouse Endoplasmic reticulum aminopeptidase 1 |
|---|---|
| Gene Symbol | ERAP1 |
| Gene Full Name | endoplasmic reticulum aminopeptidase 1 |
| Background | The protein encoded by this gene is an aminopeptidase involved in trimming HLA class I-binding precursors so that they can be presented on MHC class I molecules. The encoded protein acts as a monomer or as a heterodimer with ERAP2. This protein may also be involved in blood pressure regulation by inactivation of angiotensin II. Three transcript variants encoding two different isoforms have been found for this gene.[provided by RefSeq, Oct 2010] |
| Function | Aminopeptidase that plays a central role in peptide trimming, a step required for the generation of most HLA class I-binding peptides. Peptide trimming is essential to customize longer precursor peptides to fit them to the correct length required for presentation on MHC class I molecules. Strongly prefers substrates 9-16 residues long. Rapidly degrades 13-mer to a 9-mer and then stops. Preferentially hydrolyzes the residue Leu and peptides with a hydrophobic C-terminus, while it has weak activity toward peptides with charged C-terminus. May play a role in the inactivation of peptide hormones. May be involved in the regulation of blood pressure through the inactivation of angiotensin II and/or the generation of bradykinin in the kidney. [UniProt] |
| Cellular Localization | Endoplasmic reticulum membrane; Single-pass type II membrane protein. [UniProt] |
| Highlight | Related products: ERAP1 antibodies; ERAP1 ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | N-glycosylated. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG82731 Mouse ERAP1 ELISA Kit standard curve image
ARG82731 Mouse ERAP1 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
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