ARG82471

Mouse ENTPD2 ELISA Kit

Mouse ENTPD2 ELISA Kit for ELISA and Mouse

Component

Cat No Component Name Package Temp
ARG82471-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG82471-002 Standard 2 X 10 ng/vial 4°C
ARG82471-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG82471-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG82471-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG82471-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG82471-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG82471-008 25X Wash buffer 20 ml 4°C
ARG82471-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG82471-010 STOP solution 10 ml (Ready to use) 4°C
ARG82471-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG82471 Mouse ENTPD2 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse ENTPD2 in serum, plasma (EDTA, heparin, citrate) and cell culture supernatants.
Tested Reactivity Ms
Tested Application ELISA
Target Name ENTPD2
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 10 pg/ml
Sample Type Serum, plasma (EDTA, heparin, citrate) and cell culture supernatants.
Standard Range 15.6 - 1000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 5.1%
Inter-Assay CV: 5.5%
Alternate Names Ecto-ATPDase 2; Ecto-ATPase 2; NTPDase-2; NTPDase 2; Ecto-ATP diphosphohydrolase 2; Ectonucleoside triphosphate diphosphohydrolase 2; CD39 antigen-like 1; EC 3.6.1.-; CD39L1

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 12496 Mouse ENTPD2

Swiss-port # O55026 Mouse Ectonucleoside triphosphate diphosphohydrolase 2

Gene Symbol ENTPD2
Gene Full Name ectonucleoside triphosphate diphosphohydrolase 2
Background The protein encoded by this gene is the type 2 enzyme of the ecto-nucleoside triphosphate diphosphohydrolase family (E-NTPDase). E-NTPDases are a family of ecto-nucleosidases that hydrolyze 5'-triphosphates. This ecto-ATPase is an integral membrane protein. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jul 2008]
Function In the nervous system, could hydrolyze ATP and other nucleotides to regulate purinergic neurotransmission. Hydrolyzes ADP only to a marginal extent. The order of activity with different substrates is ATP > GTP > CTP = ITP > UTP >> ADP = UDP. [UniProt]
Cellular Localization Isoform Long: Cell membrane; Multi-pass membrane protein. Isoform Short: Endoplasmic reticulum membrane; Multi-pass membrane protein. Isoform gamma: Endoplasmic reticulum membrane; Multi-pass membrane protein. [UniProt]
Highlight Related products:

New ELISA data calculation tool:
Simplify the ELISA analysis by GainData

Images (1) Click the Picture to Zoom In

  • ARG82471 Mouse ENTPD2 ELISA Kit standard curve image

    ARG82471 Mouse ENTPD2 ELISA Kit results of a typical standard run with optical density reading at 450 nm.

Title Download Link
ARG82471 Mouse ENTPD2 ELISA Kit User manual download icon Download