ARG83039
Mouse EGF ELISA Kit
Mouse EGF ELISA Kit for ELISA and Mouse
Overview
| Product Description | ARG83039 Mouse EGF ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse EGF in serum, plasma (heparin), ascites, urine and cell culture supernatants. |
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| Tested Reactivity | Ms |
| Tested Application | ELISA |
| Specificity | Not cross-reacts with: Human EGF, HB EGF, VEGF, FGF19, PDGF BB, TGF alpha ,TGF beta1. Mouse IL6, IL8, HGF, VEGF, FGF21, GM CSF, IGF1. Porcine EGF, VEGF, TGF beta1. |
| Target Name | EGF |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Protein Full Name | Epidermal Growth Factor |
| Sensitivity | 1.95 pg/ml |
| Sample Type | Serum, plasma (heparin), ascites, urine and cell culture supernatants. |
| Standard Range | 3.9 - 250 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: less than 10% Inter-Assay CV: less than 10% |
| Alternate Names | EGF |
Application Instructions
| Assay Time | ~ 4 hours |
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Properties
| Form | 96 well |
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| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | EGF |
| Gene Full Name | Epidermal Growth Factor |
| Background | This gene encodes a member of the epidermal growth factor superfamily. The encoded preproprotein is proteolytically processed to generate the 53-amino acid epidermal growth factor peptide. This protein acts a potent mitogenic factor that plays an important role in the growth, proliferation and differentiation of numerous cell types. This protein acts by binding with high affinity to the cell surface receptor, epidermal growth factor receptor. Defects in this gene are the cause of hypomagnesemia type 4. Dysregulation of this gene has been associated with the growth and progression of certain cancers. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed. [provided by RefSeq, Jan 2016] |
| Function | EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Can induce neurite outgrowth in motoneurons of the pond snail Lymnaea stagnalis in vitro. [UniProt] |
| Cellular Localization | Membrane. [UniProt] |
| Highlight | Related products: EGF antibodies; EGF ELISA Kits; EGF Duos / Panels; EGF recombinant proteins; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | O-glycosylated with core 1-like and core 2-like glycans. It is uncertain if Ser-954 or Thr-955 is O-glycosylated. The modification here shows glycan heterogeneity: HexHexNAc (major) and Hex2HexNAc2 (minor). [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG83039 Mouse EGF ELISA Kit standard curve image
ARG83039 Mouse EGF ELISA Kit results of standard run with optical density reading at 450 nm
| Title | Download Link |
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| ARG83039 Mouse EGF ELISA Kit User manual |
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