ARG82104
Mouse EFEMP1 ELISA Kit
Mouse EFEMP1 ELISA Kit for ELISA and Mouse
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG82104-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82104-002 | Standard | 2 X 20 ng/vial | 4°C |
| ARG82104-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82104-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82104-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82104-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82104-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82104-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82104-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82104-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82104-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG82104 Mouse EFEMP1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse EFEMP1 in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Ms |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | EFEMP1 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 0.156 ng/ml |
| Sample Type | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| Standard Range | 0.312 - 20 ng/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 5.4%; Inter-Assay CV: 6.9% |
| Alternate Names | FIBL-3; MTLV; S1-5; Fibrillin-like protein; DRAD; FBNL; Extracellular protein S1-5; Fibulin-3; MLVT; FBLN3; DHRD; EGF-containing fibulin-like extracellular matrix protein 1 |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q8BPB5 Mouse EGF-containing fibulin-like extracellular matrix protein 1 |
|---|---|
| Gene Symbol | EFEMP1 |
| Gene Full Name | EGF containing fibulin-like extracellular matrix protein 1 |
| Background | This gene encodes a member of the fibulin family of extracellular matrix glycoproteins. Like all members of this family, the encoded protein contains tandemly repeated epidermal growth factor-like repeats followed by a C-terminus fibulin-type domain. This gene is upregulated in malignant gliomas and may play a role in the aggressive nature of these tumors. Mutations in this gene are associated with Doyne honeycomb retinal dystrophy. Alternatively spliced transcript variants that encode the same protein have been described.[provided by RefSeq, Nov 2009] |
| Function | Binds EGFR, the EGF receptor, inducing EGFR autophosphorylation and the activation of downstream signaling pathways. May play a role in cell adhesion and migration. May function as a negative regulator of chondrocyte differentiation. In the olfactory epithelium, it may regulate glial cell migration, differentiation and the ability of glial cells to support neuronal neurite outgrowth. [UniProt] |
| Cellular Localization | Secreted, extracellular space. Secreted, extracellular space, extracellular matrix. Note=Localizes to the lamina propria underneath the olfactory epithelium. [UniProt] |
| Highlight | Related products: EFEMP1 ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
Images (1) Click the Picture to Zoom In
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ARG82104 Mouse EFEMP1 ELISA Kit standard curve image
ARG82104 Mouse EFEMP1 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG82104 Mouse EFEMP1 ELISA Kit User's manual |
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