ARG80208
Mouse Cxcl1 ELISA Kit
Mouse Cxcl1 ELISA Kit for ELISA and Mouse
Cancer kit; Cell Biology and Cellular Response kit; Immune System kit
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG80208-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG80208-002 | Standard (Lyophilized) | 3 X 2 ng/vial | 4°C |
| ARG80208-003 | Standard diluent buffer | 20 ml | 4°C |
| ARG80208-004 | Antibody conjugate concentrate | 400 µl | 4°C |
| ARG80208-005 | Antibody diluent buffer | 16 ml | 4°C |
| ARG80208-006 | HRP-Streptavidin concentrate | 400 µl | 4°C (Protect from light) |
| ARG80208-007 | HRP-Streptavidin diluent buffer | 16 ml | 4°C |
| ARG80208-008 | 20X Wash buffer | 50 ml | 4°C |
| ARG80208-009 | TMB substrate | 12ml | 4°C (Protect from light) |
| ARG80208-010 | STOP solution | 12ml | 4°C |
| ARG80208-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG80208 Mouse Cxcl1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse Cxcl1 alpha in Serum, Plasma, Cell culture supernatants. |
|---|---|
| Tested Reactivity | Ms |
| Tested Application | ELISA |
| Specificity | No significant cross-reactivity or interference with Mouse MIP-1 alpha, MIP-1 beta, MIP-2；human GRO alpha, GRO beta, GRO gamma |
| Target Name | CXCL1 |
| Antigen Species | Mouse |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm |
| Sensitivity | 8 pg/ml |
| Sample Type | Serum, Plasma, Cell culture supernatants |
| Standard Range | 15.6 - 1000 pg/ml |
| Sample Volume | 100 µl |
| Precision | CV: less than10% |
| Alternate Names | Growth-regulated alpha protein; SCYB1; Melanoma growth stimulatory activity; MGSA-a; GRO-alpha; GROa; NAP-3; FSP; C-X-C motif chemokine 1; Neutrophil-activating protein 3; GRO1; MGSA; IL-8 homologues (murine) |
Application Instructions
| Assay Time | 4 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | Cxcl1 |
| Gene Full Name | chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha) |
| Background | This antimicrobial gene encodes a member of the CXC subfamily of chemokines. The encoded protein is a secreted growth factor that signals through the G-protein coupled receptor, CXC receptor 2. This protein plays a role in inflammation and as a chemoattractant for neutrophils. Aberrant expression of this protein is associated with the growth and progression of certain tumors. A naturally occurring processed form of this protein has increased chemotactic activity. Alternate splicing results in coding and non-coding variants of this gene. A pseudogene of this gene is found on chromosome 4. [provided by RefSeq, Sep 2014] |
| Function | Has chemotactic activity for neutrophils. May play a role in inflammation and exerts its effects on endothelial cells in an autocrine fashion. In vitro, the processed forms GRO-alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) show a 30-fold higher chemotactic activity. [UniProt] |
| Highlight | Related products: Cxcl1 antibodies; Cxcl1 ELISA Kits; Related news: Immune signaling protein TLR4 has opposing roles in breast cancer development New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| Research Area | Cancer kit; Cell Biology and Cellular Response kit; Immune System kit |
| PTM | N-terminal processed forms GRO-alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) are produced by proteolytic cleavage after secretion from peripheral blood monocytes. |
Images (1) Click the Picture to Zoom In
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ARG80208 Mouse Cxcl1 ELISA Kit standard curve image
ARG80208 Mouse Cxcl1 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG80208 Mouse Cxcl1 ELISA Kit User's manual |
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