ARG81913
Mouse Complement H ELISA Kit
Mouse Complement H ELISA Kit for ELISA and Mouse
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81913-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81913-002 | Standard | 2 X 50 ng/vial | 4°C |
| ARG81913-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81913-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81913-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81913-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81913-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81913-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81913-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81913-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81913-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81913 Mouse Complement H ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse Complement H in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Ms |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | Complement factor H |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 0.39 ng/ml |
| Sample Type | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| Standard Range | 0.78 - 50 ng/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 6.3%; Inter-Assay CV: 7.2% |
| Alternate Names | Complement factor H; ARMD4; HF1; HF2; AMBP1; ARMS1; FHL1; HUS; FH; AHUS1; H factor 1; HF; CFHL3 |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CFH |
| Gene Full Name | complement factor H |
| Background | This gene is a member of the Regulator of Complement Activation (RCA) gene cluster and encodes a protein with twenty short consensus repeat (SCR) domains. This protein is secreted into the bloodstream and has an essential role in the regulation of complement activation, restricting this innate defense mechanism to microbial infections. Mutations in this gene have been associated with hemolytic-uremic syndrome (HUS) and chronic hypocomplementemic nephropathy. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Oct 2011] |
| Function | Factor H functions as a cofactor in the inactivation of C3b by factor I and also increases the rate of dissociation of the C3bBb complex (C3 convertase) and the (C3b)NBB complex (C5 convertase) in the alternative complement pathway. [UniProt] |
| Highlight | Related products: Complement component antibodies; Complement component ELISA Kits; Complement component Duos / Panels; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
Images (1) Click the Picture to Zoom In
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ARG81913 Mouse Complement H ELISA Kit standard curve image
ARG81913 Mouse Complement H ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81913 Mouse Complement H ELISA Kit User's manual |
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