ARG81703

Mouse Cathepsin B ELISA Kit

Mouse Cathepsin B ELISA Kit for ELISA and Mouse

Component

Cat No Component Name Package Temp
ARG81703-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG81703-002 Standard 2 X 10 ng/vial 4°C
ARG81703-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG81703-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG81703-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG81703-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG81703-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG81703-008 25X Wash buffer 20 ml 4°C
ARG81703-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG81703-010 STOP solution 10 ml (Ready to use) 4°C
ARG81703-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG81703 Mouse Cathepsin B ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse Cathepsin B in serum, plasma (heparin, EDTA) and cell culture supernatants.
Tested Reactivity Ms
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name Cathepsin B
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 78 pg/ml
Sample Type Serum, plasma (heparin, EDTA) and cell culture supernatants.
Standard Range 156 - 10000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 6.5%; Inter-Assay CV: 7.5%
Alternate Names EC 3.4.22.1; Cathepsin B; APPS; CPSB; APP secretase; Cathepsin B1

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 13030 Mouse CTSB

Swiss-port # P10605 Mouse Cathepsin B

Gene Symbol CTSB
Gene Full Name cathepsin B
Background The protein encoded by this gene is a lysosomal cysteine proteinase composed of a dimer of disulfide-linked heavy and light chains, both produced from a single protein precursor. It is also known as amyloid precursor protein secretase and is involved in the proteolytic processing of amyloid precursor protein (APP). Incomplete proteolytic processing of APP has been suggested to be a causative factor in Alzheimer disease, the most common cause of dementia. Overexpression of the encoded protein, which is a member of the peptidase C1 family, has been associated with esophageal adenocarcinoma and other tumors. At least five transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
Function Thiol protease which is believed to participate in intracellular degradation and turnover of proteins. Has also been implicated in tumor invasion and metastasis. [UniProt]
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Cathepsin antibodies; Cathepsin ELISA Kits;
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Images (1) Click the Picture to Zoom In

  • ARG81703 Mouse Cathepsin B ELISA Kit standard curve image

    ARG81703 Mouse Cathepsin B ELISA Kit results of a typical standard run with optical density reading at 450 nm.

Title Download Link
ARG81703 Mouse Cathepsin B ELISA Kit User's manual download icon Download