ARG82549
Mouse Cadherin 12 ELISA Kit
Mouse Cadherin 12 ELISA Kit for ELISA and Mouse
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG82549-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82549-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82549-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82549-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82549-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82549-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82549-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82549-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82549-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82549-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82549-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG82549 Mouse Cadherin 12 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse Cadherin 12 in serum, plasma (EDTA, heparin) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Ms |
| Tested Application | ELISA |
| Target Name | Cadherin 12 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 78 pg/ml |
| Sample Type | Serum, plasma (EDTA, heparin) and cell culture supernatants. |
| Standard Range | 156 - 10000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 4.2% Inter-Assay CV: 7.1% |
| Alternate Names | Cadherin-12; Brain cadherin; CDHB; Neural type cadherin 2; BR-cadherin; N-cadherin 2 |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CDH12 |
| Gene Full Name | cadherin 12, type 2 (N-cadherin 2) |
| Background | This gene encodes a type II classical cadherin from the cadherin superfamily of integral membrane proteins that mediate calcium-dependent cell-cell adhesion. Mature cadherin proteins are composed of a large N-terminal extracellular domain, a single membrane-spanning domain, and a small, highly conserved C-terminal cytoplasmic domain. Type II (atypical) cadherins are defined based on their lack of a HAV cell adhesion recognition sequence specific to type I cadherins. This particular cadherin appears to be expressed specifically in the brain and its temporal pattern of expression would be consistent with a role during a critical period of neuronal development, perhaps specifically during synaptogenesis. [provided by RefSeq, Jul 2008] |
| Function | Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. [UniProt] |
| Cellular Localization | Cell membrane; Single-pass type I membrane protein. [UniProt] |
| Highlight | Related products: Cadherin antibodies; Cadherin ELISA Kits; Cadherin Duos / Panels; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
Images (1) Click the Picture to Zoom In
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ARG82549 Mouse Cadherin 12 ELISA Kit standard curve image
ARG82549 Mouse Cadherin 12 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG82549 Mouse Cadherin 12 ELISA Kit User manual |
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