ARG82746
Mouse CD99 ELISA Kit
Mouse CD99 ELISA Kit for ELISA and Mouse
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG82746-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82746-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82746-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82746-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82746-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82746-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82746-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82746-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82746-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82746-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82746-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG82746 Mouse CD99 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse CD99 in serum, plasma (EDTA, heparin, citrate) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Ms |
| Tested Application | ELISA |
| Target Name | CD99 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 63 pg/ml |
| Sample Type | Serum, plasma (EDTA, heparin, citrate) and cell culture supernatants. |
| Standard Range | 125 - 8000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 4.3% Inter-Assay CV: 5.9% |
| Alternate Names | 12E7; CD99 antigen; MIC2X; MIC2Y; CD antigen CD99; MSK5X; Protein MIC2; MIC2; T-cell surface glycoprotein E2; HBA71; E2 antigen |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CD99 |
| Gene Full Name | CD99 molecule |
| Background | The protein encoded by this gene is a cell surface glycoprotein involved in leukocyte migration, T-cell adhesion, ganglioside GM1 and transmembrane protein transport, and T-cell death by a caspase-independent pathway. In addition, the encoded protein may have the ability to rearrange the actin cytoskeleton and may also act as an oncosuppressor in osteosarcoma. Cyclophilin A binds to CD99 and may act as a signaling regulator of CD99. This gene is found in the pseudoautosomal region of chromosomes X and Y and escapes X-chromosome inactivation. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2013] |
| Function | Involved in T-cell adhesion processes and in spontaneous rosette formation with erythrocytes. Plays a role in a late step of leukocyte extravasation helping leukocytes to overcome the endothelial basement membrane. Acts at the same site as, but independently of, PECAM1. Involved in T-cell adhesion processes (By similarity). [UniProt] |
| Cellular Localization | Membrane; Single-pass type I membrane protein. [UniProt] |
| Highlight | Related products: CD99 antibodies; CD99 ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | Extensively O-glycosylated. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG82746 Mouse CD99 ELISA Kit standard curve image
ARG82746 Mouse CD99 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG82746 Mouse CD99 ELISA kit User manual |
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