ARG81642
Mouse CD95 / Fas ELISA Kit
Mouse CD95 / Fas ELISA Kit for ELISA and Mouse
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81642-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81642-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81642-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81642-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81642-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81642-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81642-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81642-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81642-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81642-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81642-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81642 Mouse CD95 / Fas ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse CD95 / Fas in serum, plasma (heparin, EDTA, citrate) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Ms |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | CD95 / Fas |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 15.6 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA, citrate) and cell culture supernatants. |
| Standard Range | 31.2 - 2000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 6.2%; Inter-Assay CV: 7.3% |
| Alternate Names | CD95; Apoptosis-mediating surface antigen FAS; FAS1; Tumor necrosis factor receptor superfamily member 6; ALPS1A; APT1; FASTM; CD antigen CD95; APO-1; TNFRSF6; FASLG receptor; Apo-1 antigen |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P25446 Mouse Tumor necrosis factor receptor superfamily member 6 |
|---|---|
| Gene Symbol | FAS |
| Gene Full Name | Fas cell surface death receptor |
| Background | The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor contains a death domain. It has been shown to play a central role in the physiological regulation of programmed cell death, and has been implicated in the pathogenesis of various malignancies and diseases of the immune system. The interaction of this receptor with its ligand allows the formation of a death-inducing signaling complex that includes Fas-associated death domain protein (FADD), caspase 8, and caspase 10. The autoproteolytic processing of the caspases in the complex triggers a downstream caspase cascade, and leads to apoptosis. This receptor has been also shown to activate NF-kappaB, MAPK3/ERK1, and MAPK8/JNK, and is found to be involved in transducing the proliferating signals in normal diploid fibroblast and T cells. Several alternatively spliced transcript variants have been described, some of which are candidates for nonsense-mediated mRNA decay (NMD). The isoforms lacking the transmembrane domain may negatively regulate the apoptosis mediated by the full length isoform. [provided by RefSeq, Mar 2011] |
| Function | Receptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 block apoptosis (in vitro). [UniProt] |
| Highlight | Related products: CD95 antibodies; CD95 ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | N- and O-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG81642 Mouse CD95 / Fas ELISA Kit standard curve image
ARG81642 Mouse CD95 / Fas ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81642 Mouse CD95 / Fas ELISA Kit User manual |
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