ARG81642

Mouse CD95 / Fas ELISA Kit

Mouse CD95 / Fas ELISA Kit for ELISA and Mouse

Component

Cat No Component Name Package Temp
ARG81642-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG81642-002 Standard 2 X 10 ng/vial 4°C
ARG81642-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG81642-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG81642-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG81642-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG81642-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG81642-008 25X Wash buffer 20 ml 4°C
ARG81642-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG81642-010 STOP solution 10 ml (Ready to use) 4°C
ARG81642-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG81642 Mouse CD95 / Fas ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse CD95 / Fas in serum, plasma (heparin, EDTA, citrate) and cell culture supernatants.
Tested Reactivity Ms
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name CD95 / Fas
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 15.6 pg/ml
Sample Type Serum, plasma (heparin, EDTA, citrate) and cell culture supernatants.
Standard Range 31.2 - 2000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 6.2%; Inter-Assay CV: 7.3%
Alternate Names CD95; Apoptosis-mediating surface antigen FAS; FAS1; Tumor necrosis factor receptor superfamily member 6; ALPS1A; APT1; FASTM; CD antigen CD95; APO-1; TNFRSF6; FASLG receptor; Apo-1 antigen

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 14102 Mouse FAS

Swiss-port # P25446 Mouse Tumor necrosis factor receptor superfamily member 6

Gene Symbol FAS
Gene Full Name Fas cell surface death receptor
Background The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor contains a death domain. It has been shown to play a central role in the physiological regulation of programmed cell death, and has been implicated in the pathogenesis of various malignancies and diseases of the immune system. The interaction of this receptor with its ligand allows the formation of a death-inducing signaling complex that includes Fas-associated death domain protein (FADD), caspase 8, and caspase 10. The autoproteolytic processing of the caspases in the complex triggers a downstream caspase cascade, and leads to apoptosis. This receptor has been also shown to activate NF-kappaB, MAPK3/ERK1, and MAPK8/JNK, and is found to be involved in transducing the proliferating signals in normal diploid fibroblast and T cells. Several alternatively spliced transcript variants have been described, some of which are candidates for nonsense-mediated mRNA decay (NMD). The isoforms lacking the transmembrane domain may negatively regulate the apoptosis mediated by the full length isoform. [provided by RefSeq, Mar 2011]
Function Receptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 block apoptosis (in vitro). [UniProt]
Highlight Related products:
CD95 antibodies; CD95 ELISA Kits;
New ELISA data calculation tool:
Simplify the ELISA analysis by GainData
PTM N- and O-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans. [UniProt]

Images (1) Click the Picture to Zoom In

  • ARG81642 Mouse CD95 / Fas ELISA Kit standard curve image

    ARG81642 Mouse CD95 / Fas ELISA Kit results of a typical standard run with optical density reading at 450 nm.

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