ARG81717
Mouse CD40 ELISA Kit
Mouse CD40 ELISA Kit for ELISA and Mouse
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81717-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81717-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81717-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81717-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81717-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81717-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81717-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81717-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81717-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81717-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81717-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81717 Mouse CD40 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse CD40 in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Ms |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | CD40 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 7.8 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| Standard Range | 15.6 - 1000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 5.2%; Inter-Assay CV: 6.3% |
| Alternate Names | CDw40; CD antigen CD40; Tumor necrosis factor receptor superfamily member 5; Bp50; CD40L receptor; CDW40; TNFRSF5; p50; B-cell surface antigen CD40 |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P27512 Mouse Tumor necrosis factor receptor superfamily member 5 |
|---|---|
| Gene Symbol | CD40 |
| Gene Full Name | CD40 molecule, TNF receptor superfamily member 5 |
| Background | This gene is a member of the TNF-receptor superfamily. The encoded protein is a receptor on antigen-presenting cells of the immune system and is essential for mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. AT-hook transcription factor AKNA is reported to coordinately regulate the expression of this receptor and its ligand, which may be important for homotypic cell interactions. Adaptor protein TNFR2 interacts with this receptor and serves as a mediator of the signal transduction. The interaction of this receptor and its ligand is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis. Mutations affecting this gene are the cause of autosomal recessive hyper-IgM immunodeficiency type 3 (HIGM3). Multiple alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Nov 2014] |
| Function | Receptor for TNFSF5/CD40LG. Transduces TRAF6- and MAP3K8-mediated signals that activate ERK in macrophages and B cells, leading to induction of immunoglobulin secretion. [UniProt] |
| Highlight | Related products: CD40 antibodies; CD40 ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
Images (1) Click the Picture to Zoom In
-
ARG81717 Mouse CD40 ELISA Kit standard curve image
ARG81717 Mouse CD40 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81717 Mouse CD40 ELISA Kit User's manual |
 Download
|
