ARG81717

Mouse CD40 ELISA Kit

Mouse CD40 ELISA Kit for ELISA and Mouse

Component

Cat No Component Name Package Temp
ARG81717-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG81717-002 Standard 2 X 10 ng/vial 4°C
ARG81717-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG81717-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG81717-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG81717-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG81717-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG81717-008 25X Wash buffer 20 ml 4°C
ARG81717-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG81717-010 STOP solution 10 ml (Ready to use) 4°C
ARG81717-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG81717 Mouse CD40 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse CD40 in serum, plasma (heparin, EDTA) and cell culture supernatants.
Tested Reactivity Ms
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name CD40
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 7.8 pg/ml
Sample Type Serum, plasma (heparin, EDTA) and cell culture supernatants.
Standard Range 15.6 - 1000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 5.2%; Inter-Assay CV: 6.3%
Alternate Names CDw40; CD antigen CD40; Tumor necrosis factor receptor superfamily member 5; Bp50; CD40L receptor; CDW40; TNFRSF5; p50; B-cell surface antigen CD40

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 21939 Mouse CD40

Swiss-port # P27512 Mouse Tumor necrosis factor receptor superfamily member 5

Gene Symbol CD40
Gene Full Name CD40 molecule, TNF receptor superfamily member 5
Background This gene is a member of the TNF-receptor superfamily. The encoded protein is a receptor on antigen-presenting cells of the immune system and is essential for mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. AT-hook transcription factor AKNA is reported to coordinately regulate the expression of this receptor and its ligand, which may be important for homotypic cell interactions. Adaptor protein TNFR2 interacts with this receptor and serves as a mediator of the signal transduction. The interaction of this receptor and its ligand is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis. Mutations affecting this gene are the cause of autosomal recessive hyper-IgM immunodeficiency type 3 (HIGM3). Multiple alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Nov 2014]
Function Receptor for TNFSF5/CD40LG. Transduces TRAF6- and MAP3K8-mediated signals that activate ERK in macrophages and B cells, leading to induction of immunoglobulin secretion. [UniProt]
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Images (1) Click the Picture to Zoom In

  • ARG81717 Mouse CD40 ELISA Kit standard curve image

    ARG81717 Mouse CD40 ELISA Kit results of a typical standard run with optical density reading at 450 nm.

Title Download Link
ARG81717 Mouse CD40 ELISA Kit User's manual download icon Download