ARG81685
Mouse CD30 ELISA Kit
Mouse CD30 ELISA Kit for ELISA and Mouse
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81685-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81685-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81685-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81685-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81685-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81685-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81685-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81685-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81685-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81685-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81685-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81685 Mouse CD30 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse CD30 in serum and cell culture supernatants. |
|---|---|
| Tested Reactivity | Ms |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | CD30 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 7.8 pg/ml |
| Sample Type | Serum and cell culture supernatants. |
| Standard Range | 15.6 - 1000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 5.8%; Inter-Assay CV: 7.2% |
| Alternate Names | Tumor necrosis factor receptor superfamily member 8; Ki-1 antigen; CD30; Ki-1; Lymphocyte activation antigen CD30; CD antigen CD30; D1S166E; CD30L receptor |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q60846 Mouse Tumor necrosis factor receptor superfamily member 8 |
|---|---|
| Gene Symbol | TNFRSF8 |
| Gene Full Name | tumor necrosis factor receptor superfamily, member 8 |
| Background | CD30 is a member of the TNF-receptor superfamily. This receptor is expressed by activated, but not by resting, T and B cells. TRAF2 and TRAF5 can interact with this receptor, and mediate the signal transduction that leads to the activation of NF-kappaB. This receptor is a positive regulator of apoptosis, and also has been shown to limit the proliferative potential of autoreactive CD8 effector T cells and protect the body against autoimmunity. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008] |
| Function | CD30 is a receptor for TNFSF8/CD30L (PubMed:8391931). May play a role in the regulation of cellular growth and transformation of activated lymphoblasts. Regulates gene expression through activation of NF-kappa-B (PubMed:8999898). [UniProt] |
| Highlight | Related products: CD30 antibodies; CD30 ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | Phosphorylated on serine and tyrosine residues. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG81685 Mouse CD30 ELISA Kit standard curve image
ARG81685 Mouse CD30 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81685 Mouse CD30 ELISA Kit User's manual |
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