ARG81711
Mouse CD14 ELISA Kit
Mouse CD14 ELISA Kit for ELISA and Mouse
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81711-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81711-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81711-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81711-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81711-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81711-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81711-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81711-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81711-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81711-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81711-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81711 Mouse CD14 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse CD14 in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Ms |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | CD14 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 0.39 ng/ml |
| Sample Type | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| Standard Range | 0.78 - 50 ng/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 5.4%; Inter-Assay CV: 6.0% |
| Alternate Names | CD antigen CD14; Myeloid cell-specific leucine-rich glycoprotein; Monocyte differentiation antigen CD14 |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P10810 Mouse Monocyte differentiation antigen CD14 |
|---|---|
| Gene Symbol | CD14 |
| Gene Full Name | CD14 molecule |
| Background | The protein encoded by this gene is a surface antigen that is preferentially expressed on monocytes/macrophages. It cooperates with other proteins to mediate the innate immune response to bacterial lipopolysaccharide. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Mar 2010] |
| Function | In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the MD-2/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules. [UniProt] |
| Highlight | Related products: CD14 antibodies; CD14 ELISA Kits; CD14 Duos / Panels; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | N- and O- glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG81711 Mouse CD14 ELISA Kit standard curve image
ARG81711 Mouse CD14 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81711 Mouse CD14 ELISA Kit User's manual |
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