ARG81971
Mouse CD117 / c-Kit ELISA Kit
Mouse CD117 / c-Kit ELISA Kit for ELISA and Mouse
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81971-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81971-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81971-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81971-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81971-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81971-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81971-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81971-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81971-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81971-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81971-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81971 Mouse CD117 / c-Kit ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse CD117 / c-Kit in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Ms |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | CD117 / c-Kit |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 78 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| Standard Range | 156 - 10000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 6.1%; Inter-Assay CV: 7.3% |
| Alternate Names | PBT; C-Kit; Tyrosine-protein kinase Kit; CD antigen CD117; Mast/stem cell growth factor receptor Kit; CD117; Proto-oncogene c-Kit; SCFR; Piebald trait protein; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog; p145 c-kit; EC 2.7.10.1 |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P05532 Mouse Mast/stem cell growth factor receptor Kit |
|---|---|
| Gene Symbol | KIT |
| Gene Full Name | v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog |
| Background | This gene encodes the human homolog of the proto-oncogene c-kit. C-kit was first identified as the cellular homolog of the feline sarcoma viral oncogene v-kit. This protein is a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). Mutations in this gene are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous lukemia, and piebaldism. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| Function | Tyrosine-protein kinase that acts as cell-surface receptor for the cytokine KITLG/SCF and plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. In response to KITLG/SCF binding, KIT can activate several signaling pathways. Phosphorylates PIK3R1, PLCG1, SH2B2/APS and CBL. Activates the AKT1 signaling pathway by phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase. Activated KIT also transmits signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. Promotes activation of STAT family members STAT1, STAT3, STAT5A and STAT5B. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KIT signaling is modulated by protein phosphatases, and by rapid internalization and degradation of the receptor. Activated KIT promotes phosphorylation of the protein phosphatases PTPN6/SHP-1 and PTPRU, and of the transcription factors STAT1, STAT3, STAT5A and STAT5B. Promotes phosphorylation of PIK3R1, CBL, CRK (isoform Crk-II), LYN, MAPK1/ERK2 and/or MAPK3/ERK1, PLCG1, SRC and SHC1. [UniProt] |
| Highlight | Related products: CD117 antibodies; CD117 ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | Ubiquitinated by SOCS6. KIT is rapidly ubiquitinated after autophosphorylation induced by KITLG/SCF binding, leading to internalization and degradation. Autophosphorylated on tyrosine residues. KITLG/SCF binding enhances autophosphorylation. Isoform 1 shows low levels of tyrosine phosphorylation in the absence of added KITLG/SCF (in vitro). Kinase activity is down-regulated by phosphorylation on serine residues by protein kinase C family members. Phosphorylation at Tyr-568 is required for interaction with PTPN11/SHP-2, CRK (isoform Crk-II) and members of the SRC tyrosine-protein kinase family. Phosphorylation at Tyr-570 is required for interaction with PTPN6/SHP-1. Phosphorylation at Tyr-703, Tyr-823 and Tyr-936 is important for interaction with GRB2. Phosphorylation at Tyr-721 is important for interaction with PIK3R1. Phosphorylation at Tyr-823 and Tyr-936 is important for interaction with GRB7. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG81971 Mouse CD117 / c-Kit ELISA Kit standard curve image
ARG81971 Mouse CD117 / c-Kit ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81971 Mouse CD117 / c-Kit ELISA Kit User manual |
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