ARG81677
Mouse CD106 / VCAM1 ELISA Kit
Mouse CD106 / VCAM1 ELISA Kit for ELISA and Mouse
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81677-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81677-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81677-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81677-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81677-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81677-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81677-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81677-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81677-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81677-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81677-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81677 Mouse CD106 / VCAM1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse CD106 / VCAM1 in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Ms |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | CD106 / VCAM1 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 78 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| Standard Range | 156 - 10000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 6.9%; Inter-Assay CV: 8.2% |
| Alternate Names | CD106; INCAM-100; Vascular cell adhesion protein 1; VCAM-1; CD antigen CD106; V-CAM 1 |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | VCAM1 |
| Gene Full Name | vascular cell adhesion molecule 1 |
| Background | This gene is a member of the Ig superfamily and encodes a cell surface sialoglycoprotein expressed by cytokine-activated endothelium. This type I membrane protein mediates leukocyte-endothelial cell adhesion and signal transduction, and may play a role in the development of artherosclerosis and rheumatoid arthritis. Three alternatively spliced transcripts encoding different isoforms have been described for this gene. [provided by RefSeq, Dec 2010] |
| Function | Important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with integrin alpha-4/beta-1 (ITGA4/ITGB1) on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/ITGA4/ITGB1 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation. [UniProt] |
| Highlight | Related products: CD106 antibodies; CD106 ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | Sialoglycoprotein. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG81677 Mouse CD106 / VCAM1 ELISA Kit standard curve image
ARG81677 Mouse CD106 / VCAM1 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81677 Mouse CD106 / VCAM1 ELISA Kit User's manual |
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