ARG81677

Mouse CD106 / VCAM1 ELISA Kit

Mouse CD106 / VCAM1 ELISA Kit for ELISA and Mouse

Component

Cat No Component Name Package Temp
ARG81677-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG81677-002 Standard 2 X 10 ng/vial 4°C
ARG81677-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG81677-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG81677-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG81677-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG81677-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG81677-008 25X Wash buffer 20 ml 4°C
ARG81677-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG81677-010 STOP solution 10 ml (Ready to use) 4°C
ARG81677-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG81677 Mouse CD106 / VCAM1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse CD106 / VCAM1 in serum, plasma (heparin, EDTA) and cell culture supernatants.
Tested Reactivity Ms
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name CD106 / VCAM1
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 78 pg/ml
Sample Type Serum, plasma (heparin, EDTA) and cell culture supernatants.
Standard Range 156 - 10000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 6.9%; Inter-Assay CV: 8.2%
Alternate Names CD106; INCAM-100; Vascular cell adhesion protein 1; VCAM-1; CD antigen CD106; V-CAM 1

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 22329 Mouse VCAM1

Swiss-port # P29533 Mouse Vascular cell adhesion protein 1

Gene Symbol VCAM1
Gene Full Name vascular cell adhesion molecule 1
Background This gene is a member of the Ig superfamily and encodes a cell surface sialoglycoprotein expressed by cytokine-activated endothelium. This type I membrane protein mediates leukocyte-endothelial cell adhesion and signal transduction, and may play a role in the development of artherosclerosis and rheumatoid arthritis. Three alternatively spliced transcripts encoding different isoforms have been described for this gene. [provided by RefSeq, Dec 2010]
Function Important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with integrin alpha-4/beta-1 (ITGA4/ITGB1) on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/ITGA4/ITGB1 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation. [UniProt]
Highlight Related products:
CD106 antibodies; CD106 ELISA Kits;
New ELISA data calculation tool:
Simplify the ELISA analysis by GainData
PTM Sialoglycoprotein. [UniProt]

Images (1) Click the Picture to Zoom In

  • ARG81677 Mouse CD106 / VCAM1 ELISA Kit standard curve image

    ARG81677 Mouse CD106 / VCAM1 ELISA Kit results of a typical standard run with optical density reading at 450 nm.

Title Download Link
ARG81677 Mouse CD106 / VCAM1 ELISA Kit User's manual download icon Download