ARG82081
Mouse CCL19 / MIP-3 beta ELISA Kit
Mouse CCL19 / MIP-3 beta ELISA Kit for ELISA and Mouse
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG82081-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82081-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82081-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82081-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82081-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82081-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82081-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82081-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82081-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82081-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82081-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG82081 Mouse CCL19 / MIP-3 beta ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse CCL19 / MIP-3 beta in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Ms |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | CCL19 / MIP-3 beta |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 7.8 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| Standard Range | 15.6 - 1000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 5.7%; Inter-Assay CV: 6.8% |
| Alternate Names | Small-inducible cytokine A19; EBI1 ligand chemokine; CKb11; SCYA19; ELC; MIP-3b; MIP3B; C-C motif chemokine 19; Macrophage inflammatory protein 3 beta; Epstein-Barr virus-induced molecule 1 ligand chemokine; Beta-chemokine exodus-3; CK beta-11; MIP-3-beta |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CCL19 |
| Gene Full Name | chemokine (C-C motif) ligand 19 |
| Background | This antimicrobial gene is one of several CC cytokine genes clustered on the p-arm of chromosome 9. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The cytokine encoded by this gene may play a role in normal lymphocyte recirculation and homing. It also plays an important role in trafficking of T cells in thymus, and in T cell and B cell migration to secondary lymphoid organs. It specifically binds to chemokine receptor CCR7. [provided by RefSeq, Sep 2014] |
| Function | May play a role not only in inflammatory and immunological responses but also in normal lymphocyte recirculation and homing. May play an important role in trafficking of T-cells in thymus, and T-cell and B-cell migration to secondary lymphoid organs. Binds to chemokine receptor CCR7. Recombinant CCL19 shows potent chemotactic activity for T-cells and B-cells but not for granulocytes and monocytes. Binds to atypical chemokine receptor ACKR4 and mediates the recruitment of beta-arrestin (ARRB1/2) to ACKR4. [UniProt] |
| Cellular Localization | Secreted. [UniProt] |
| Highlight | Related products: CCL19 antibodies; CCL19 ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
Images (1) Click the Picture to Zoom In
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ARG82081 Mouse CCL19 / MIP-3 beta ELISA Kit standard curve image
ARG82081 Mouse CCL19 / MIP-3 beta ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG82081 Mouse CCL19 / MIP-3 beta ELISA Kit User's manual |
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