ARG81976
Mouse CA9 / Carbonic Anhydrase 9 ELISA Kit
Mouse CA9 / Carbonic Anhydrase 9 ELISA Kit for ELISA and Mouse
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81976-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81976-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81976-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81976-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81976-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81976-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81976-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81976-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81976-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81976-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81976-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81976 Mouse CA9 / Carbonic Anhydrase 9 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse CA9 / Carbonic Anhydrase 9 in serum, plasma (heparin, EDTA, citrate), urine and cell culture supernatants. |
|---|---|
| Tested Reactivity | Ms |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | CA9 / Carbonic Anhydrase 9 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 7.8 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA, citrate), urine and cell culture supernatants. |
| Standard Range | 15.6 - 1000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 6.8%; Inter-Assay CV: 7.6% |
| Alternate Names | EC 4.2.1.1; MN; CAIX; pMW1; Carbonic anhydrase 9; Carbonic anhydrase IX; RCC-associated antigen G250; Membrane antigen MN; P54/58N; Carbonate dehydratase IX; Renal cell carcinoma-associated antigen G250; CA-IX |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | CA9 |
|---|---|
| Gene Full Name | carbonic anhydrase IX |
| Background | Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA IX is a transmembrane protein and is one of only two tumor-associated carbonic anhydrase isoenzymes known. It is expressed in all clear-cell renal cell carcinoma, but is not detected in normal kidney or most other normal tissues. It may be involved in cell proliferation and transformation. This gene was mapped to 17q21.2 by fluorescence in situ hybridization, however, radiation hybrid mapping localized it to 9p13-p12. [provided by RefSeq, Jun 2014] |
| Function | Reversible hydration of carbon dioxide. Participates in pH regulation. May be involved in the control of cell proliferation and transformation. Appears to be a novel specific biomarker for a cervical neoplasia. [UniProt] |
| Highlight | Related products: Carbonic Anhydrase antibodies; Carbonic Anhydrase ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | Asn-346 bears high-mannose type glycan structures. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG81976 Mouse CA9 / Carbonic Anhydrase 9 ELISA Kit standard curve image
ARG81976 Mouse CA9 / Carbonic Anhydrase 9 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81976 Mouse CA9 / Carbonic Anhydrase 9 ELISA Kit User's manual |
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