ARG82112
Mouse BMP10 ELISA Kit
Mouse BMP10 ELISA Kit for ELISA and Mouse
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG82112-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82112-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82112-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82112-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82112-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82112-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82112-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82112-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82112-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82112-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82112-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG82112 Mouse BMP10 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse BMP10 in serum, plasma (heparin, EDTA, citrate) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Ms |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | BMP10 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 7.8 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA, citrate) and cell culture supernatants. |
| Standard Range | 15.6 - 1000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 4.9%; Inter-Assay CV: 6.3% |
| Alternate Names | Bone morphogenetic protein 10; BMP-10 |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | BMP10 |
| Gene Full Name | bone morphogenetic protein 10 |
| Background | The protein encoded by this gene is a member of the TGF-beta family of growth factors. Data suggest that the similar protein in mouse plays an important role in trabeculation of the embryonic heart. In human, this protein may signal through receptor serine/threonine kinases. [provided by RefSeq, Jul 2008] |
| Function | Required for maintaining the proliferative activity of embryonic cardiomyocytes by preventing premature activation of the negative cell cycle regulator CDKN1C/p57KIP and maintaining the required expression levels of cardiogenic factors such as MEF2C and NKX2-5. Acts as a ligand for ACVRL1/ALK1, BMPR1A/ALK3 and BMPR1B/ALK6, leading to activation of SMAD1, SMAD5 and SMAD8 transcription factors. Inhibits endothelial cell migration and growth. May reduce cell migration and cell matrix adhesion in breast cancer cell lines. [UniProt] |
| Cellular Localization | Secreted. [UniProt] |
| Highlight | Related products: BMP antibodies; BMP ELISA Kits; BMP recombinant proteins; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
Images (1) Click the Picture to Zoom In
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ARG82112 Mouse BMP10 ELISA Kit standard curve image
ARG82112 Mouse BMP10 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG82112 Mouse BMP10 ELISA Kit User's manual |
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