ARG83226
Mouse BID ELISA Kit
Mouse BID ELISA Kit for ELISA and Mouse
Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody; Apoptosis Marker antibody; Pro-apoptotic Bcl2 protein antibody
Overview
| Product Description | ARG83226 Mouse BID ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse BID in Serum, Plasma and Cell culture supernatants. |
|---|---|
| Tested Reactivity | Ms |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | Bid |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 15 pg/ml |
| Detection Range | 31.2 pg/ml - 2,000 pg/ml |
| Sample Type | Serum, Plasma and Cell culture supernatants |
| Precision | Intra-Assay CV: 5.2% Inter-Assay CV: 5.5% |
| Alternate Names | p22 BID; BID; p15 BID; p11 BID; BH3-interacting domain death agonist; p13 BID; FP497 |
Application Instructions
| Assay Time | ~ 5 hours |
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Properties
| Form | 96 well |
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| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P70444 Mouse BH3-interacting domain death agonist |
|---|---|
| Gene Symbol | BID |
| Gene Full Name | BH3 interacting domain death agonist |
| Background | This gene encodes a death agonist that heterodimerizes with either agonist BAX or antagonist BCL2. The encoded protein is a member of the BCL-2 family of cell death regulators. It is a mediator of mitochondrial damage induced by caspase-8 (CASP8); CASP8 cleaves this encoded protein, and the COOH-terminal part translocates to mitochondria where it triggers cytochrome c release. Multiple alternatively spliced transcript variants have been found, but the full-length nature of some variants has not been defined. |
| Highlight | Related Antibody Duos and Panels: ARG30275 Pro-apoptotic Bcl2 protein Antibody Panel (BAX, BAK, Bid) Related products: Bid antibodies; Bid Duos / Panels; Anti-Rabbit IgG secondary antibodies; |
| Research Area | Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody; Apoptosis Marker antibody; Pro-apoptotic Bcl2 protein antibody |
| PTM | TNF-alpha induces a caspase-mediated cleavage of p22 BID into a major p15 and minor p13 and p11 products. p15 BID is ubiquitinated by ITCH; ubiquitination results in proteasome-dependent degradation. |
Images (1) Click the Picture to Zoom In
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ARG83226 Mouse BID ELISA Kit standard curve image
ARG83226 Mouse BID ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
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| ARG83226 Mouse BID ELISA Kit User manual |
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