ARG82077
Mouse Activin A ELISA Kit
Mouse Activin A ELISA Kit for ELISA and Mouse
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG82077-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82077-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82077-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82077-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82077-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82077-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82077-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82077-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82077-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82077-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82077-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG82077 Mouse Activin A ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse Activin A in serum, plasma (heparin, EDTA), saliva and cell culture supernatants. |
|---|---|
| Tested Reactivity | Ms |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | Activin A |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 7.8 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA), saliva and cell culture supernatants. |
| Standard Range | 15.6 - 1000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 6.5%; Inter-Assay CV: 6.5% |
| Alternate Names | Erythroid differentiation protein; Activin beta-A chain; FRP; EDF; Inhibin beta A chain |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | INHBA |
| Gene Full Name | inhibin, beta A |
| Background | The inhibin beta A subunit joins the alpha subunit to form a pituitary FSH secretion inhibitor. Inhibin has been shown to regulate gonadal stromal cell proliferation negatively and to have tumor-suppressor activity. In addition, serum levels of inhibin have been shown to reflect the size of granulosa-cell tumors and can therefore be used as a marker for primary as well as recurrent disease. Because expression in gonadal and various extragonadal tissues may vary severalfold in a tissue-specific fashion, it is proposed that inhibin may be both a growth/differentiation factor and a hormone. Furthermore, the beta A subunit forms a homodimer, activin A, and also joins with a beta B subunit to form a heterodimer, activin AB, both of which stimulate FSH secretion. Finally, it has been shown that the beta A subunit mRNA is identical to the erythroid differentiation factor subunit mRNA and that only one gene for this mRNA exists in the human genome. [provided by RefSeq, Jul 2008] |
| Function | Inhibins and activins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins. [UniProt] |
| Cellular Localization | Secreted. [UniProt] |
| Highlight | Related products: Activin A antibodies; Activin A ELISA Kits; Activin A recombinant proteins; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
Images (1) Click the Picture to Zoom In
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ARG82077 Mouse Activin A ELISA Kit standard curve image
ARG82077 Mouse Activin A ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG82077 Mouse Activin A ELISA Kit User's manual |
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