ARG81679
Mouse ACE ELISA Kit
Mouse ACE ELISA Kit for ELISA and Mouse
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81679-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81679-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81679-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81679-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81679-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81679-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81679-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81679-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81679-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81679-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81679-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81679 Mouse ACE ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse ACE in serum, plasma (heparin) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Ms |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | ACE |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 46.9 pg/ml |
| Sample Type | Serum, plasma (heparin) and cell culture supernatants. |
| Standard Range | 93.8 - 6000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 5.9%; Inter-Assay CV: 6.6% |
| Alternate Names | DCP1; ICH; ACE; EC 3.2.1.-; MVCD3; Angiotensin-converting enzyme; Dipeptidyl carboxypeptidase I; CD143; CD antigen CD143; EC 3.4.15.1; Kininase II; ACE1; DCP |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | ACE |
| Gene Full Name | angiotensin I converting enzyme |
| Background | This gene encodes an enzyme involved in catalyzing the conversion of angiotensin I into a physiologically active peptide angiotensin II. Angiotensin II is a potent vasopressor and aldosterone-stimulating peptide that controls blood pressure and fluid-electrolyte balance. This enzyme plays a key role in the renin-angiotensin system. Many studies have associated the presence or absence of a 287 bp Alu repeat element in this gene with the levels of circulating enzyme or cardiovascular pathophysiologies. Multiple alternatively spliced transcript variants encoding different isoforms have been identified, and two most abundant spliced variants encode the somatic form and the testicular form, respectively, that are equally active. [provided by RefSeq, May 2010] |
| Function | Converts angiotensin I to angiotensin II by release of the terminal His-Leu, this results in an increase of the vasoconstrictor activity of angiotensin. Also able to inactivate bradykinin, a potent vasodilator. Has also a glycosidase activity which releases GPI-anchored proteins from the membrane by cleaving the mannose linkage in the GPI moiety. [UniProt] |
| Highlight | Related products: ACE antibodies; ACE ELISA Kits; ACE Duos / Panels; ACE recombinant proteins; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | Phosphorylated by CK2 on Ser-1299; which allows membrane retention. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG81679 Mouse ACE ELISA Kit standard curve image
ARG81679 Mouse ACE ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81679 Mouse ACE ELISA Kit User's manual |
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