ARG82770

MPO / Myeloperoxidase Activity Assay Kit (Colorimetric)

MPO / Myeloperoxidase Activity Assay Kit (Colorimetric) for Functional study and Human,Mouse,Rat,All

publication_link Publication2

Overview

Product Description ARG82770 MPO / Myeloperoxidase Activity Assay Kit (Colorimetric) can be used to measure Myeloperoxidase activity in whole neutrophils, neutrophil lysates, tissue homogenates and plasma (EDTA).
Tested Reactivity Hu, Ms, Rat, All
Tested Application FuncSt
Target Name MPO / Myeloperoxidase
Conjugation Note Read at 405 nm (colorimetric)
Sensitivity 4.2 µM (0.63 nmol/well, based on 150 µl volume/well)
Detection Range 4.2 - 333 µM (0.63 - 50 nmol/well, based on 150 µl volume/well)
Sample Type Whole neutrophils, neutrophil lysates, tissue homogenates and plasma (EDTA).
Sample Volume 25 µl
Alternate Names MPO; Myeloperoxidase; EC 1.11.2.2

Application Instructions

Application Note Please note that this kit does not include a microplate.
Assay Time ~ 2.5 hours

Properties

Form Liquid
Storage Instruction Store the kit at 2-8°C. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 17523 Mouse MPO

GeneID: 4353 Human MPO

Swiss-port # P05164 Human Myeloperoxidase

Swiss-port # P11247 Mouse Myeloperoxidase

Gene Symbol MPO
Gene Full Name myeloperoxidase
Background Myeloperoxidase (MPO) is a heme protein synthesized during myeloid differentiation that constitutes the major component of neutrophil azurophilic granules. Produced as a single chain precursor, myeloperoxidase is subsequently cleaved into a light and heavy chain. The mature myeloperoxidase is a tetramer composed of 2 light chains and 2 heavy chains. This enzyme produces hypohalous acids central to the microbicidal activity of neutrophils. [provided by RefSeq, Nov 2014]
Function Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity. [UniProt]
Cellular Localization Lysosome. [UniProt]

Images (1) Click the Picture to Zoom In

  • ARG82770 MPO / Myeloperoxidase Activity Assay Kit (Colorimetric) typical data demonstration image

    ARG82770 MPO / Myeloperoxidase Activity Assay Kit (Colorimetric) results of a typical standard run with optical density reading at 405 nm, using colorimetric reading.

Specific References

Pioglitazone protects against acetic acid –induced ulcerative colitis in rats via modulation of SIRT1 / NOX4 signaling and suppression of macrophage M1 polarization

Functional Assay / 

Soad L Kabil et al.
Zagazig University Medical Journal,  (2024)

publication_link

 

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Hypochlorous Acid Chemistry in Mammalian Cells-Influence on Infection and Role in Various Pathologies

Functional Assay / 

Celia María Curieses Andrés et al.
Int J Mol Sci.,  (2022)

publication_link

 

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Title Download Link
ARG82770 MPO / Myeloperoxidase Activity Assay Kit (Colorimetric) User's manual download icon Download