ARG82770
MPO / Myeloperoxidase Activity Assay Kit (Colorimetric)
MPO / Myeloperoxidase Activity Assay Kit (Colorimetric) for Functional study and Human,Mouse,Rat,All species
Publication1
Overview
| Product Description | ARG82770 MPO / Myeloperoxidase Activity Assay Kit (Colorimetric) can be used to measure Myeloperoxidase activity in whole neutrophils, neutrophil lysates, tissue homogenates and plasma (EDTA). |
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| Tested Reactivity | Hu, Ms, Rat, All |
| Tested Application | FuncSt |
| Target Name | MPO / Myeloperoxidase |
| Conjugation Note | Read at 405 nm (colorimetric) |
| Sensitivity | 4.2 µM (0.63 nmol/well, based on 150 µl volume/well) |
| Detection Range | 4.2 - 333 µM (0.63 - 50 nmol/well, based on 150 µl volume/well) |
| Sample Type | Whole neutrophils, neutrophil lysates, tissue homogenates and plasma (EDTA). |
| Sample Volume | 25 µl |
| Alternate Names | MPO; Myeloperoxidase; EC 1.11.2.2 |
Application Instructions
| Application Note | Please note that this kit does not include a microplate. |
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| Assay Time | ~ 2.5 hours |
Properties
| Form | Liquid |
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| Storage Instruction | Store the kit at 2-8°C. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | MPO |
| Gene Full Name | myeloperoxidase |
| Background | Myeloperoxidase (MPO) is a heme protein synthesized during myeloid differentiation that constitutes the major component of neutrophil azurophilic granules. Produced as a single chain precursor, myeloperoxidase is subsequently cleaved into a light and heavy chain. The mature myeloperoxidase is a tetramer composed of 2 light chains and 2 heavy chains. This enzyme produces hypohalous acids central to the microbicidal activity of neutrophils. [provided by RefSeq, Nov 2014] |
| Function | Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity. [UniProt] |
| Cellular Localization | Lysosome. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG82770 MPO / Myeloperoxidase Activity Assay Kit (Colorimetric) typical data demonstration image
ARG82770 MPO / Myeloperoxidase Activity Assay Kit (Colorimetric) results of a typical standard run with optical density reading at 405 nm, using colorimetric reading.
Specific References
Hypochlorous Acid Chemistry in Mammalian Cells-Influence on Infection and Role in Various Pathologies
Functional Assay /
| Title | Download Link |
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| ARG82770 MPO / Myeloperoxidase Activity Assay Kit (Colorimetric) User's manual |
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