ARG80804
Lupin ELISA Kit
Lupin ELISA Kit for ELISA
Immune System kit
Publication1
Overview
| Product Description | ARG80804 Lupin ELISA Kit is a Enzyme Immunoassay kit for the quantification of Lupin in food (extraction, dilution). |
|---|---|
| Tested Application | ELISA |
| Target Name | Lupin |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm |
| Sensitivity | 0.2 ppm |
| Sample Type | Food (extraction, dilution). |
| Standard Range | 2 - 30 ppm |
| Sample Volume | 100 µl |
Application Instructions
| Assay Time | 20, 20 min (RT), 20 min (RT/dark) |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Full Name | Lupin |
|---|---|
| Background | Lupin belongs to the legume family. With about 35-45% the fraction of proteins in lupin seed is very high. Some of these proteins, predominantly those in the alpha, beta and gamma-Conglutin fractions are known for being allergenic, due to cross-reaction to allergens of soy and peanut. Because of its convenient nutritional properties the use of lupin flour as a food ingredient is highly increasing. Lupin is often used in dietary products, especially for milk allergic people, vegetarians and patients with celiac disease. In food production three (sweet) lupin species are commonly used: Lupinus albus, Lupinus angustifolius and Lupinus luteus. For lupin allergic persons hidden lupin allergens in food are a critical problem. Already very low amounts of lupin can cause allergic reactions, which may lead to anaphylactic shock in severe cases. Because of this, lupin allergic persons must strictly avoid the consumption of lupin containing food. Cross-contamination, mostly in consequence of the production process, is often noticed. This explains why in many cases the existence of lupin residues in food cannot be excluded. For this reason sensitive detection systems for lupin residues in foodstuffs are required. The Lupin ELISA represents a highly sensitive detection system for lupin and is particularly capable of the quantification of residues in sausage, bakery products, potato products, ketchup and juices. Due to high cross-reactivity the test is suitable for the detection of all commonly used lupin species. |
| Highlight | Related products: New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| Research Area | Immune System kit |
Images (1) Click the Picture to Zoom In
-
ARG80804 Lupin ELISA Kit standard curve image
ARG80804 Lupin ELISA Kit results of a typical standard run with optical density reading at 450nm.
Specific References
Lupine allergens: Clinical relevance, molecular characterization, cross-reactivity, and detection strategies
(Review) / (Review)
| Title | Download Link |
|---|---|
| ARG80804 Lupin ELISA Kit User's manual |
 Download
|
