ARG81196
Irisin ELISA Kit
Irisin ELISA Kit for ELISA and Human,Mouse,Rat,Dog
Publication2
Overview
| Product Description | ARG81196 Irisin ELISA Kit is a Competitive Enzyme Immunoassay kit for the quantification of Irisin in serum and plasma. |
|---|---|
| Tested Reactivity | Hu, Ms, Rat, Dog |
| Tested Application | ELISA |
| Specificity | Irisin (Human, Mouse, Rat, Canine): 100% Irisin (aa. 42-112) (Human, Mouse, Rat, Canine): 100% Irisin, Recombinant (Human, Mouse, Rat, Canine): 100% FNDC5 (Isoform 4) (Human, Mouse, Rat): 9% Irisin precursor, C-terminal / FNDC5 (aa. 165 - 212) (Human) / FNDC5 (aa. 162-209) (Mouse, Rat): 0% Irisin (aa. 42-95) (Human, Mouse, Rat, Canine): 0% |
| Target Name | Irisin |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm |
| Sensitivity | 6.2 ng/ml |
| Detection Range | Linear Range: 6.2 - 205 ng/ml |
| Sample Type | Serum and plasma. |
| Standard Range | 0.1 - 1000 ng/ml |
| Sample Volume | 50 µl |
| Precision | Intra-Assay CV: < 10% Inter-Assay CV: < 15% |
| Alternate Names | irisin; FRCP2; Fibronectin type III domain-containing protein 5; Fibronectin type III repeat-containing protein 2 |
Application Instructions
| Assay Time | ~ 5 hours |
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Properties
| Form | 96 well |
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| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | FNDC5 |
| Gene Full Name | fibronectin type III domain containing 5 |
| Background | This gene encodes a secreted protein that is released from muscle cells during exercise. The encoded protein may participate in the development of brown fat. Translation of the precursor protein initiates at a non-AUG start codon at a position that is conserved as an AUG start codon in other organisms. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2013] |
| Function | Irisin: Contrary to mouse, may not be involved in the beneficial effects of muscular exercise, nor in the induction of browning of human white adipose tissue. [UniProt] |
| Highlight | Related products: Irisin antibodies; Irisin ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | The extracellular domain is cleaved and released from the cell membrane. N-Glycosylated. |
Images (1) Click the Picture to Zoom In
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ARG81196 Irisin ELISA Kit standard curve image
ARG81196 Irisin ELISA Kit results of a typical standard run with optical density reading at 450 nm.
Specific References
Polysaccharides from Enteromorpha prolifera improves insulin sensitivity and promotes adipose thermogenesis in diet-induced obese mice associated with activation of PGC-1α-FNDC5/irisin pathway
ELISA / Mouse / Serum
Curcumin improves insulin sensitivity and increases energy expenditure in high-fat-diet-induced obese mice associated with activation of FNDC5/irisin
ELISA / Mouse
| Title | Download Link |
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| ARG81196 Irisin ELISA Kit User's manual |
 Download
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