ARG81195
Irisin ELISA Kit
Irisin ELISA Kit for ELISA and Human,Mouse,Rat,Dog
Overview
| Product Description | ARG81195 Irisin ELISA Kit is a Competitive Enzyme Immunoassay kit for the quantification of Irisin in plasma (EDTA) and serum samples. |
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| Tested Reactivity | Hu, Ms, Rat, Dog |
| Tested Application | ELISA |
| Specificity | Irisin, Recombinant (Human, Rat, Mouse, Dog): 100% Irisin (aa. 1-112) (Human, Mouse, Rat, Dog): 100% FNDC5, isoform 4 recombinant (Human, Mouse, Rat, Dog): 9% Irisin precursor, C-terminal 48mer: Human FNDC5 (aa. 165-212); Mouse, Rat FNDC5 (aa. 162-209): 0% Irisin (aa. 42-95) (Human, Mouse, Rat, Dog): 0% |
| Target Name | Irisin |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm |
| Sensitivity | 2.62 ng/ml |
| Detection Range | 0.1 - 1000 ng/ml |
| Sample Type | plasma (EDTA) and serum |
| Standard Range | 0.1 - 1000 ng/ml |
| Sample Volume | 50 µl |
| Alternate Names | irisin; FRCP2; Fibronectin type III domain-containing protein 5; Fibronectin type III repeat-containing protein 2 |
Application Instructions
| Assay Time | ~ 5 hours |
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Properties
| Form | 96 well |
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| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | FNDC5 |
| Gene Full Name | fibronectin type III domain containing 5 |
| Background | This gene encodes a secreted protein that is released from muscle cells during exercise. The encoded protein may participate in the development of brown fat. Translation of the precursor protein initiates at a non-AUG start codon at a position that is conserved as an AUG start codon in other organisms. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2013] |
| Function | Irisin: Contrary to mouse, may not be involved in the beneficial effects of muscular exercise, nor in the induction of browning of human white adipose tissue. [UniProt] |
| Highlight | Related products: Irisin antibodies; Irisin ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | The extracellular domain is cleaved and released from the cell membrane. N-Glycosylated. |
Images (1) Click the Picture to Zoom In
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ARG81195 Irisin ELISA Kit standard curve image
ARG81195 Irisin ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
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| ARG81195 Irisin ELISA Kit User's manual |
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