ARG81192
Intracellular ROS Assay Kit (Fluorometric)
Intracellular ROS Assay Kit (Fluorometric) for Functional study and All
Publication1
Overview
| Tested Reactivity | All |
|---|---|
| Tested Application | FuncSt |
| Target Name | ROS |
| Conjugation | Un-conjugated |
| Conjugation Note | Read the fluorescence with a fluorescence plate reader at 480 nm excitation / 530 nm emission. |
| Sensitivity | 0.01 nM |
| Sample Type | Cultured cells |
| Standard Range | 0.01 - 10000 nM |
| Sample Volume | Cultured cells in either a clear or black 96-well cell culture plate. |
Application Instructions
| Assay Time | ~ 1 hour |
|---|
Properties
| Form | Liquid |
|---|---|
| Storage Instruction | Store components at 4°C or -20°C. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Images (3) Click the Picture to Zoom In
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ARG81192 Intracellular ROS Assay Kit (Fluorometric) standard curve image
ARG81192 Intracellular ROS Assay Kit (Fluorometric) results of a typical standard run with optical density reading at 480 nm excitation / 530 nm emission.
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ROS in HeLa cells treated with H2O2
50,000 HeLa cells in a 96-well plate were first pretreated with 1 mM DCFH-DA for 60 minutes at 37°C. Cells were then treated with various concentrations of H2O2 for 20 minutes.
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DCF Fluorescence in HeLa cells treated with H2O2
DCF Fluorescence in H2O2 treated HeLa cells after 1 hour. Left: 0 µM; Right: 1000 µM.
Specific References
TNFAIP3 interacting protein 2 relieves lipopolysaccharide (LPS)-induced inflammatory injury in endometritis by inhibiting NF-kappaB activation
FuncSt / Human
| Title | Download Link |
|---|---|
| ARG81192 Intracellular ROS Assay Kit (Fluorometric) User's manual |
 Download
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